Objective: The aim of the study is to examine parents' knowledge and awareness regarding the routine vaccination of children. Study design: The research was conducted as a cross-sectional study. Subjects and methods: The participants were parents of children who attended the pediatric clinic of Dr. Sonja Mihalec. The total number of participants was 115. The questionnaire was filled out in person during the parents' visits to the pediatric clinic. Inclusion criteria were all the children who were patients of Dr. Sonja Mihalec's pediatric clinic, while exclusion criteria were all the other children who did not belong to the pediatric clinic and those admitted as emergency cases. Results: A total of 115 participants were included in the study, consisting of the child's mother, father, and guardians. The median age was 36 years. Most participants have moderate or good knowledge of the types of vaccines but rarely check vaccination information and consult with the pediatrician. Trust in the safety and effectiveness of routine vaccination varies, with many participants considering the information to be partially accessible and reliable. Most parents believe it is important to ensure vaccination according to the schedule, but doubts and the need for better education are present. Based on the results, improving the accessibility and comprehensibility of vaccination information is crucial for strengthening parents' trust. Conclusion: The results of this study indicate that there is significant room for improving parents' awareness and education about children's vaccination. Although most parents have basic knowledge and a positive attitude towards the importance of vaccination, they do not check information or consult with health professionals frequently enough. Improving the availability and comprehensibility of information and strengthening trust in the health system can contribute to greater safety and effectiveness of children's vaccination.