The policy of Habsburgs towards the spread of the Reformation in the Holy Roman Empire was discussed as the topic of this paper. The order of Charles V on the complete destruction of schisms and sects from 1550, the Religious peace of Augsburg from 1555 and the Peace of Westphalia from 1648 were used as primary sources in writing of this paper. Early modern history of the Holy Roman Empire began with the coronation of Maximilian I Habsburg as emperor. During the reign of Emperor Maximilian I of Habsburg, the Habsburgs began to turn into a great power. The year 1517 can be taken as the beginning of the Reformation, when Martin Luther nailed his text against sale of pardons to the door of the Wittenberg church. All classes understood Luther`s theses in different ways. Luther also enjoyed the support of the nobility and masses. The emperor of the Holy Roman Empire, Charles V, turned against the Reformation, and by means of the Edict of Worms from 1521, Luther was declared a heretic and a exile. In 1555, after the war between the nobles who supported Refomation and the emperor, the Augsburg Peace was concluded, which confirmed the right of the nobles to determine the religion of their subjects. At the beginning, the church reacted midly to the Reformation. Later, it`s reaction escalated and Luther, together with his supporters, was officially excommunicated from the Church by papal bull in 1521. The Refomation spread through the Holy Roman Empire thanks to nobility and the press. The policy of Habsburgs towards the spread of the Reformation was of changing character. Namely, during the time of Charles V and Ferdinand I, it was mostly of a repressive nature, while during the time of Ferdinand`s sons, Maximilian II and Charles VI was of conciliatory nature. The repressive policy occured again during the reign of emperor Rudolf II and Ferdinand II. The tolerant policy towards the Refomation and its spread came during reign of emperor Ferdinand III.