This study examined the relationship between the Big Five personality traits and movie genre preferences among Croatian student respondents. The Big Five traits of 203 participants were measured as well as their level of enjoyment of eleven different film genres namely: Thriller, Romance, Western, Comedy, Action, Drama, Science Fiction, Crime, Horror, Fantasy, and Musical. Other than personality traits, factors as their movie-watching frequency, whether they identify as movie lovers/enthusiasts or not, their enjoyment of happy and realistic endings in movies, and their enjoyment of watching movies alone and in the company of others were considered. Several significant associations were found between movie genre preferences and the Big Five. Specifically, agreeableness, conscientiousness, and openness were positively linked to a preference for thrillers, while extraversion and agreeableness were associated with a preference for romance. Comedy enjoyment was positively related to both agreeableness and conscientiousness, while the preference for drama with the traits of extraversion, agreeableness, conscientiousness, and openness. Openness was also positively linked to a preference for science fiction and fantasy. The enjoyment of Crime was positively associated with openness and agreeableness. Additionally, preferences for musicals were positively associated with agreeableness and extraversion. After the correlation analysis, 11 regression analyses in which additional measures of movie-watching preferences were added. Our regression analysis showed that the predictor openness showed a significant positive relationship and the predictor drawn to happy endings a significant negative relationship with the enjoyment of Thrillers. The predictors of agreeableness and drawn to happy endings both showed a strong and significant positive relationship with the enjoyment of Romance. Openness showed a significant negative relationship with the enjoyment of Romance. The predictor gender showed a significant positive relationship with the enjoyment of Westerns.The predictors drawn to happy endings and agreeableness showed a significant positive relationship with the enjoyment of Comedy. None of the predictors showed a statistically significant relationship with the enjoyment of Action. The predictor drawn to realistic endings showed a positive relationship with the enjoyment of Drama. The predictor enjoying watching alone showed a very strong positive relationship with the enjoyment of Drama. Movie-watching frequency and drawn to happy endings showed a negative relationship with the enjoyment of Drama. Openness and Emotional stability showed a positive relationship with the enjoyment of Science Fiction. The predictors conscientiousness and drawn to realistic endings showed a significant negative relationship with the enjoyment of Science Fiction. The predictor movie lover showed a positive relationship with the enjoyment of Crime and drawn to happy endings a negative relationship with the enjoyment of Crime. The predictors of gender and movie-watching frequency showed a significant positive relationship with the enjoyment of Horror. Drawn to happy endings showed a significant negative relationship with the enjoyment of Horror. Openness showed a significant positive relationship with the enjoyment of Fantasy. Drawn to realistic endings showed a significant negative relationship with the enjoyment of Fantasy. Drawn to happy endings showed a strong significant positive relationship with the enjoyment of Musicals. The predictor extraversion showed a significant positive relationship with the enjoyment of musicals. The predictors of emotional stability and conscientiousness showed a significant negative relationship with the enjoyment of Musicals. Thus, these findings contribute to the understanding of how personality differences affect media choice. The practical implications of these findings can be useful to content creators, marketers, and recommendation systems about tailoring movie recommendations to users’ personalities to enhance viewer satisfaction and engagement.