The aim of the study was to examine gender differences in the perception of a person's humor, as well as to examine the role of physical attractiveness and humor when assessing readiness for various forms of romantic relationships, depending on the participant's gender. An online experimental study was conducted with 314 students from different Croatian universities. To examine differences in humor perception, we analyzed the results of male and female participants (N = 312), and for readiness for different types of relationships, we analyzed the results of heterosexual participants only (N = 254). Physical attractiveness was manipulated through photographs of individuals who were either of high or low physical attractiveness, and humor was manipulated through a series of humorous or neutral statements accompanying the photographs that did not differ in previously assessed humor. Participants completed an online questionnaire in which they provided evaluations of the individuals on multiple characteristics, including humor and readiness for five different types of romantic relationships with them, varying in seriousness and duration. Men rated the statements as funnier when they believed they were made by a man rather than by a woman. In contrast, women rated men and women as equally humorous. For men, humor influenced readiness only for long-term relationships, where they expressed greater willingness to enter a relationship with humorous women, but only if they were also physically attractive. Women expressed greater readiness for a one-time romantic encounter, a long-term relationship, and marriage with more humorous partners, but only if they were physically attractive. However, they expressed greater willingness for going on a few dates and dating with humorous individuals, for both high and low attractiveness men. However, both men and women expressed low readiness for any form of relationship.