The research in this paper focuses on the analysis of male and female characters in the tragicomedies of Junije Palmotić from a gender perspective. At the beginning, a summary of the relevant studies of Palmotić's dramas so far is presented, after which the theoretical starting points of the research are explained. The analysis is based on gender theory on the one hand, and on the other on the starting points of character analysis in contemporary drama theory. The interpretation of Palmotić's plays also takes into account the genre conventions of Dubrovnik's tragicomedy. The research hypothesis aims to prove that the protagonists in Palmotić's tragicomedies were shaped according to genre conventions and at the same time in accordance with the early modern patriarchal worldview and its harmonized gender roles. The research interprets seven dramas, two mythological dramas (Elena ugrabjena and Ipsipile), two dramas created according to the templates of the Italian Renaissance epic (Alčina and Armida) and three pseudohistorical tragicomedies – Captislava, Danica and Pavlimir. The conclusion draws a synthetic review of what was established by the analysis, referring to the main textual and genre strategies through which Palmotić's tragicomedies reproduce and promote the ideals of masculinity and femininity characteristic of the early modern period.