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master's thesis
Imobilizacija aldolaze na magnetske nanočestice
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2019. urn:nbn:hr:149:106823
Rimac, Tomislav
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Rimac, T. (2019). Imobilizacija aldolaze na magnetske nanočestice (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:106823

Rimac, Tomislav. "Imobilizacija aldolaze na magnetske nanočestice." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2019. https://urn.nsk.hr/urn:nbn:hr:149:106823

Rimac, Tomislav. "Imobilizacija aldolaze na magnetske nanočestice." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2019. https://urn.nsk.hr/urn:nbn:hr:149:106823

Rimac, T. (2019). 'Imobilizacija aldolaze na magnetske nanočestice', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 17 June 2024, https://urn.nsk.hr/urn:nbn:hr:149:106823

Rimac T. Imobilizacija aldolaze na magnetske nanočestice [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2019 [cited 2024 June 17] Available at: https://urn.nsk.hr/urn:nbn:hr:149:106823

T. Rimac, "Imobilizacija aldolaze na magnetske nanočestice", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:149:106823

Metadata
TitleImobilizacija aldolaze na magnetske nanočestice
Title (english)Immobilisation of aldolase on magnetic nanoparticle
AuthorTomislav Rimac
MentorAna Vrsalović Presečki (mentor)
Committee memberAna Vrsalović Presečki (predsjednik povjerenstva)
Committee memberAna Lončarić Božić (član povjerenstva)
Committee memberMarin Kovačić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2019-07-12, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
Enzim deoksiriboza-5-fosfat aldolaza (DERA) katalizira reakciju sinteze (3R,5R)-6-kloro-3,5- dihidroksi heksanala (laktol) dvostrukom aldolnom adicijom dvije molekule acetaldehida i jedne molekule kloroacetaldehida. Visoka koncentracija aldehida u DERA reakciji ima izrazito negativan utjecaj na stabilnost enzima. Cilj ovog rada bio je provesti imobilizaciju enzima DERA-e na magnetske nosioce u svrhu povećavanja njegove stabilnosti. Kao nosioci za imobilizaciju korištene su magnetske nanočestice obložene neporoznom i mezoporoznom silikom. Nosioci su aktivirani s benzokinonom, glutaraldehidom i epoksi skupinom. Najveća stabilnost i aktivnost dobivena je imobilizacijom enzima DERA-e na magnetske nanočestice obložene neporoznom silikom aktivirane benzokinonom.
Abstract (english)
Deoxyribose-5-phosphate aldolase (DERA) catalyses the synthesis of (3R,5R)-6-chloro-3,5-dihidroxyhexanal (lactol) by double aldol addition of two molecules of acetaldehyde and one molecule of chloroacetaldehyde. High aldehyde concentrations in DERA reaction have significant negative impact on enzyme stability. The aim of this paper was to carry out the immobilization of DERA enzyme on magnetic carriers in order to increase its stability. Magnetic nanoparticles coated with mesoporous and nonporous silica were used as the immobilisation carriers. Carriers were activated by benzoquinone, glutaraldehyde and epoxy group. The highest stability and activity was achieved by immobilising DERA enzyme onto nanomagnetic particles coated with nonporous silica and activated by benzoquinone.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:106823
Study programmeTitle: Environmental Engineering - Graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2019-07-12
Terms of useLicence In copyright icon
Created on2022-02-27 14:40:13