In this paper, the effect of the addition of TiO2 nanoparticles of a range of 1% wt. to 5% wt. on the structure and properties of PA 2200 polymer matrix was investigated. Polymer nanocomposites were prepared by melting the polymer and mixing the nanoparticles using the Brabender plasticoder and Fontune hydraulic press. The characterization of the samples was performed using differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), tenisle test and thermal conductivity using THB method. DSC analysis shows that TiO2 nanofiller improve the polymer matrix crystallization and increase the crystallization temperature of the polymer matrix by acting as nucleation centres. Additionally, TiO2 nanofiller contribute to the formation of more stable crystal structures. TGA analysis showed that addition of TiO2 nanoparticles shifts the temperature at the beginning of degradation toward higher values, therefore increasing thermal stability of PA matrix. Mechanical properties determined by tensile stress test, imply that by the addition of the TiO2 nanofiller, the secant modulus is reduced. Additionally, slight changes in yield stress and yield strain relative to the pure polymer matrix were observed. The addition of TiO2 nanoparticles increases the thermal conductivity of a nanocomposite relative to a pure polymer matrix.