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master's thesis
Razvoj stabilitetno-indikativne UHPLC metode za određivanje farmaceutski aktivnog spoja i njegovih srodnih spojeva
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2019. urn:nbn:hr:149:624697
Bukač, Kristina
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Bukač, K. (2019). Razvoj stabilitetno-indikativne UHPLC metode za određivanje farmaceutski aktivnog spoja i njegovih srodnih spojeva (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:624697

Bukač, Kristina. "Razvoj stabilitetno-indikativne UHPLC metode za određivanje farmaceutski aktivnog spoja i njegovih srodnih spojeva." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2019. https://urn.nsk.hr/urn:nbn:hr:149:624697

Bukač, Kristina. "Razvoj stabilitetno-indikativne UHPLC metode za određivanje farmaceutski aktivnog spoja i njegovih srodnih spojeva." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2019. https://urn.nsk.hr/urn:nbn:hr:149:624697

Bukač, K. (2019). 'Razvoj stabilitetno-indikativne UHPLC metode za određivanje farmaceutski aktivnog spoja i njegovih srodnih spojeva', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 20 December 2024, https://urn.nsk.hr/urn:nbn:hr:149:624697

Bukač K. Razvoj stabilitetno-indikativne UHPLC metode za određivanje farmaceutski aktivnog spoja i njegovih srodnih spojeva [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2019 [cited 2024 December 20] Available at: https://urn.nsk.hr/urn:nbn:hr:149:624697

K. Bukač, "Razvoj stabilitetno-indikativne UHPLC metode za određivanje farmaceutski aktivnog spoja i njegovih srodnih spojeva", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:149:624697

Metadata
TitleRazvoj stabilitetno-indikativne UHPLC metode za određivanje farmaceutski aktivnog spoja i njegovih srodnih spojeva
Title (english)Development of stability-indicating UHPLC method for the determination of active pharmaceutical ingredient and its related substances
AuthorKristina Bukač
MentorSandra Babić (mentor)
Committee memberSandra Babić (predsjednik povjerenstva)
Committee memberŠime Ukić (član povjerenstva)
Committee memberLidija Furač (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2019-11-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U ovom radu opisan je razvoj metode za analizu farmaceutski aktivne tvari cediraniba, njegovih onečišćenja i razgradnih produkata. Razvijena je metoda tekućinske kromatografije ultravisoke djelotvornosti (UHPLC) s detektorom s nizom dioda (DAD) s C18 kromatografskom kolonom i gradijentnim eluiranjem. Optimalni eksperimentalni uvjeti analize određeni su primjenom računalnog programa Drylab: gradijentno eluiranje u trajanju od 15 minuta, temperatura kolone 60 °C i pH-vrijednost pokretne faze 5,8. Provedena je prisilna razgradnja cediraniba u lužnatom i kiselom mediju, te pomoću okidativnog sredstva. Za analizu cediraniba i razgradnih produkata korištena je metoda UHPLC i metoda dvodimenzionalne UHPLC, obje vezane sa spektrometrijom masa visoke razlučivosti (spektrometar masa koji mjeri vrijeme preleta, QTOF). Na temelju dobivenih spektara mase i točnih masa određenih pomoću QTOF-a, pretpostavljene su strukture razgradnih produkata i način fragmentacije.
Abstract (english)
This work describes the development of an analytical method for the determination of active pharmaceutical ingredient cediranib, its impurities and degradation products using ultra-high performance liquid chromatography (UHPLC) with diode-array detector (DAD), C18 stationary phase and gradient elution. Optimal experimental conditions were determined by using computer software DryLab®: gradient elution with a run time of 15 minutes, column temperature 60 °C and pH-value of mobile phase 5,8. Influence of pH and the presence of oxidants on the degradation of cediranib active substance was investigated. Cediranib and degradation impurities were identified using ultra-high performance liquid chromatography and two-dimensional liquid chromatography coupled to high resolution mass spectrometry (quadrupole time of flight mass spectrometer, QTOF). The structures of degradation products were proposed based on mass spectra and exact masses determined by QTOF.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:624697
Study programmeTitle: Applied Chemistry - Graduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry
Course: Applied organic chemistry
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra primijenjene kemije (magistar/magistra primijenjene kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-01-01
Terms of useLicence In copyright icon
Created on2022-02-28 17:28:12