Advances in chiral technology and the ability to produce enantiomerically pure compounds have an important impact on drug design, research, and development. In this paper, the sensitivity analysis of the parameters on the cascade reaction model is performed. The investigated cascade reaction consists of three reaction steps: i. opening of (S)-epoxide and (R)-epoxide with cyanate ions, ii. opening of (S)-epoxide and (R)-epoxide with bromide ions, iii. closing the dibromoalcohol ring. The first two steps were performed in the presence of the enzyme halohydrin dehalogenase. The sensitivity analysis of the parameters was performed for the model of kinetic resolution and dynamic kinetic resolution, ie a series of simulations of the mathematical model were performed. Since the maximum yield at kinetic resolution is only 50 % and it is quite difficult to separate the optically active substrate and product, simulations were performed for the model of dynamic kinetic resolution, ie with the addition of bromide ions. Graphical representations of the dependence of the deviation of the optical purity (ee) from the zero ee value on the value of an individual parameter were obtained. The influence of the value of an individual parameter on ee at kinetic resolution was observed at 48 % substrate conversion, while at dynamic kinetic resolution it was observed at 100 % substrate conversion. All examined kinetic parameters have an influence on the change of the deviation ee from the zero value, some in the whole examined area, and some only in a part of the examined area.