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master's thesis
Optimiranje uvjeta imobilizacije aldolaze na mezoporoznu siliku
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2020. urn:nbn:hr:149:269554
Kolić, Barbara
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

Cite this document

Kolić, B. (2020). Optimiranje uvjeta imobilizacije aldolaze na mezoporoznu siliku (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:269554

Kolić, Barbara. "Optimiranje uvjeta imobilizacije aldolaze na mezoporoznu siliku." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2020. https://urn.nsk.hr/urn:nbn:hr:149:269554

Kolić, Barbara. "Optimiranje uvjeta imobilizacije aldolaze na mezoporoznu siliku." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2020. https://urn.nsk.hr/urn:nbn:hr:149:269554

Kolić, B. (2020). 'Optimiranje uvjeta imobilizacije aldolaze na mezoporoznu siliku', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 17 June 2024, https://urn.nsk.hr/urn:nbn:hr:149:269554

Kolić B. Optimiranje uvjeta imobilizacije aldolaze na mezoporoznu siliku [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2020 [cited 2024 June 17] Available at: https://urn.nsk.hr/urn:nbn:hr:149:269554

B. Kolić, "Optimiranje uvjeta imobilizacije aldolaze na mezoporoznu siliku", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:149:269554

Metadata
TitleOptimiranje uvjeta imobilizacije aldolaze na mezoporoznu siliku
Title (english)Optimisation of aldolase immobilisation on mesoporous silica
AuthorBarbara Kolić
MentorAna Vrsalović Presečki (mentor)
Committee memberAna Vrsalović Presečki (predsjednik povjerenstva)
Committee memberAna Lončarić Božić (član povjerenstva)
Committee memberMarin Kovačić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2020-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
U ovom radu provedena je imobilizacija enzima DERA na MCF mezoporoznu siliku u puferima koncetracija 0,05, 0,1 i 0,2 M pri pH vrijednostima 6, 6,5, 7, 7,5 i 8. MCF mezoporozna silika sintetizirana je sol-gel procesom. Aktivnost enzima ispitana je u reakciji aldolne adicije acetaldehida i kloroacetaldehida. Ovom reakcijom nastaje laktol, prekursor za sintezu bočnog lanca statina. Usporedbom parametara uspješnosti za navedene pufere odabrani su optimalni uvjeti za imobilizaciju enzima koji su odgovarali puferu koncentracije 0,05 M i pH vrijednosti 6,5. Imobilizacija enzima DERA potom je ispitana za nosioc većih pora u odabranom puferu. Usporedbom parametara uspješnosti oba nosioca, za nosioc većih pora dobivene su više vrijednosti efikasnosti i preostale aktivnosti.
Abstract (english)
In this work enzyme DERA was immobilized on MCF mesoporous silica in various immobilization buffers. Buffers with different ionic strenght, i.e. concentration (0.05, 0.1 and 0.2 M) and pH (6, 6.5, 7, 7.5 and 8) were used. MCF mesoporous silica was synthesized by sol-gel process. Enzyme activity was measured in the reaction of aldol addition of acetaldehyde and chloroacetaldehyde. The product of this reaction is lactol, the precursor in synthesis of a statin side chain. Based on the measure of success of immobilization process, optimal immobilization buffer was the one with concentration of 0.5 M and pH 6.5. Further, the enzyme DERA was immobilized on the MCF carrier with larger pores in the optimal buffer. Higher values of immobilization efficiency and remaining activity were obtained for the carrier with larger pores.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:269554
Study programmeTitle: Environmental Engineering - Graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2021-07-01
Terms of useLicence In copyright icon
Created on2022-03-05 09:49:09