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master's thesis
Ispitivanje utjecaja procesa aktivacije nosioca na efikasnost imobilizacije
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2020. urn:nbn:hr:149:832279
Punčec, Marina
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Punčec, M. (2020). Ispitivanje utjecaja procesa aktivacije nosioca na efikasnost imobilizacije (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:832279

Punčec, Marina. "Ispitivanje utjecaja procesa aktivacije nosioca na efikasnost imobilizacije." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2020. https://urn.nsk.hr/urn:nbn:hr:149:832279

Punčec, Marina. "Ispitivanje utjecaja procesa aktivacije nosioca na efikasnost imobilizacije." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2020. https://urn.nsk.hr/urn:nbn:hr:149:832279

Punčec, M. (2020). 'Ispitivanje utjecaja procesa aktivacije nosioca na efikasnost imobilizacije', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 17 June 2024, https://urn.nsk.hr/urn:nbn:hr:149:832279

Punčec M. Ispitivanje utjecaja procesa aktivacije nosioca na efikasnost imobilizacije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2020 [cited 2024 June 17] Available at: https://urn.nsk.hr/urn:nbn:hr:149:832279

M. Punčec, "Ispitivanje utjecaja procesa aktivacije nosioca na efikasnost imobilizacije", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:149:832279

Metadata
TitleIspitivanje utjecaja procesa aktivacije nosioca na efikasnost imobilizacije
Title (english)Investigation of carrier activation on the immobilisation efficiency
AuthorMarina Punčec
MentorAna Vrsalović Presečki (mentor)
Committee memberAna Vrsalović Presečki (predsjednik povjerenstva)
Committee memberAna Lončarić Božić (član povjerenstva)
Committee memberMarin Kovačić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2020-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
DERA (deoksiriboza-5-fosfat aldolaza) je enzim iz skupine aldolaza, izoliran iz životinjskog tkiva i mikroorganizama. Katalizira reakciju sinteze (3R, 5R)-6-kloro-3,5-dihidroksi heksanala (laktola), prekursora u proizvodnji statina, aldolnom adicijom dvije molekule acetaldehida i jedne molekule kloroacetaldehida. U navedenoj reakciji, znatno je izražena deaktivacija enzima, uzrokovana visokim koncentracijama aldehida u sustavu. Cilj ovog rada bio je imobilizirati enzim DERA-u na magnetske nanočestice obložene silikom u svrhu povećanja njezine stabilnosti u prisustvu aldehida, a zatim ispitati utjecaj procesa aktivacije nosioca na efikasnost imobilizacije. Ispitan je utjecaj dvaju reagensa za aktivaciju nosioca: benzokinona i sukcinid anhidrida, te temperature i pH - parametara koji imaju znatan utjecaj na proces imobilizacije. Prilikom imobilizacije, kao najbolje sredstvo za aktivaciju pokazao se benzokinon, a najveća efikasnost imobilizacije enzima DERA-e je postignuta pri temperaturi od 20 °C i pH 6.
Abstract (english)
DERA (deoxyribose-5-phosphate aldolase) is an enzyme from the aldolase group, isolated from animal tissue and microorganisms. It catalyses the synthesis of statin precursor, (3R, 5R) -6-chloro-3,5-dihydroxy hexanal (lactol) in the reaction of the aldol addition of two acetaldehyde and one chloroacetaldehyde. In this reaction, enzyme deactivation is significant and it's caused by high concentrations of aldehydes in the system. The aim of this work was to immobilise the enzyme DERA on silica-coated magnetic nanoparticles in order to increase its stability in the presence of aldehydes, and to examine the impact of the carrier activation process on the immobilisation efficiency. The influence of two activation agents was investigated: benzoquinone and succinic anhydride, as well as temperature and pH, which have a significant impact on immobilisation process. During immobilisation, benzoquinone proved to be the best activation agent, and the the highest efficiency of immobilisation process was achieved at the temperature of 20 °C and pH 6.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:832279
Study programmeTitle: Chemical Engineering - Graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2021-07-01
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Created on2022-03-07 16:59:23