prikaz prve stranice dokumenta Biokatalitička redukcija acetaldehida
  Download
PDF 1006.25 KB
undergraduate thesis
Biokatalitička redukcija acetaldehida
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. urn:nbn:hr:149:288656
Bukarica, Ivana
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

Cite this document

Bukarica, I. (2018). Biokatalitička redukcija acetaldehida (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:288656

Bukarica, Ivana. "Biokatalitička redukcija acetaldehida." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:149:288656

Bukarica, Ivana. "Biokatalitička redukcija acetaldehida." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:149:288656

Bukarica, I. (2018). 'Biokatalitička redukcija acetaldehida', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 22 March 2025, https://urn.nsk.hr/urn:nbn:hr:149:288656

Bukarica I. Biokatalitička redukcija acetaldehida [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2018 [cited 2025 March 22] Available at: https://urn.nsk.hr/urn:nbn:hr:149:288656

I. Bukarica, "Biokatalitička redukcija acetaldehida", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:149:288656

Metadata
TitleBiokatalitička redukcija acetaldehida
Title (english)Biocatalytical reduction of acetaldehyde
AuthorIvana Bukarica
MentorAna Vrsalović Presečki (mentor)
Committee memberAna Vrsalović Presečki (predsjednik povjerenstva)
Committee memberVanja Kosar (član povjerenstva)
Committee memberDajana Kučić Grgić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2018-09-19, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Reaction Engineering
Abstract
U ovom radu ispitivana je kinetika reakcije redukcije acetaldehida uz biokatalizator ketoreduktazu koja za svoju aktivnost zahtijeva uporabu koenzima nikotinamid-adenin dinukleotid fosfata. Kinetika je mjerena metodom početnih brzina variranjem koncentracija supstrata (acetaldehida) i koenzima (NADPH) te su procijenjeni kinetički parametri Michaelis-Menteničine kinetike. Ispitivan je i utjecaj produkata na brzinu reakcije, te aktivnost enzima u povratnoj reakciji oksidacije etanola. Mjerenja su... More pokazala da produkt NADP+ inhibira reakciju, te da enzim ne pokazuje aktivnost u reakciji oksidacije etanola. Također je izmjerena stabilnost biokatalizatora pri različitim koncentracijama acetaldehida, te se pokazalo da povećanje koncentracije acetaldehida ima negativan utjecaj na stabilnost enzima. Na osnovu kinetičkih mjerenja i mjerenja stabilnosti biokatalizatora postavljen je matematički model redukcije acetaldehida u kotlastom reaktoru, te su napravljene simulacije procesa. Less
Abstract (english)
In this paper the kinetics of acetaldehyde reduction catalysed by an ketoreductase was investigated. For its activity, ketoreductase demands the use of coenzyme nicotinamide-adenine dinucleotide phosphate. The kinetics was measured by determining the initial reaction rate by changing the concentrations of substrate (acetaldehyde) and coenzyme (NADPH). These data were used for the estimation of the kinetic parameters of Michaelis-Menten equation. The influence of products on the reaction rate,... More and the activity of enzyme in the reverse reaction, oxidation of ethanol, was also examined. Measurements have shown that the product NADP+ inhibits the reaction, and that the enzyme doesn't show activity in the reaction of ethanol oxidation. Stability of enzyme was measured in the presence of different concentrations of acetaldehyde, and it was shown that increase of acetaldehyde concentration have negative effect on the stability of the enzyme. Based on experimental data and the enzyme stability measurements, mathematical model was set for the reaction of reduction of acetaldehyde in the batch reactor, and simulations of the process have been done. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:288656
ProjectNumber: 635595
Title: Sustainable industrial processes based on a C-C bond-forming enzyme platform
Acronym: CARBAZYMES
Leader: Zvjezdana Findrik Blažević
Jurisdiction: eu
Funder: EC
Funding stream: H2020
Study programmeTitle: Environmental Engineering - Undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-05-20 13:06:27
accessibility

closeAccessibilityrefresh

If you wish to permanently save changes, click on Save, if not - your settings will be reset when you restart the browser.