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undergraduate thesis
Modeliranje biokatalitičke redukcije acetona katalizirane ketoreduktazom
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. urn:nbn:hr:149:522510
Pohanić, Petra
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Pohanić, P. (2018). Modeliranje biokatalitičke redukcije acetona katalizirane ketoreduktazom (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:522510

Pohanić, Petra. "Modeliranje biokatalitičke redukcije acetona katalizirane ketoreduktazom." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:149:522510

Pohanić, Petra. "Modeliranje biokatalitičke redukcije acetona katalizirane ketoreduktazom." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:149:522510

Pohanić, P. (2018). 'Modeliranje biokatalitičke redukcije acetona katalizirane ketoreduktazom', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 09 November 2024, https://urn.nsk.hr/urn:nbn:hr:149:522510

Pohanić P. Modeliranje biokatalitičke redukcije acetona katalizirane ketoreduktazom [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2018 [cited 2024 November 09] Available at: https://urn.nsk.hr/urn:nbn:hr:149:522510

P. Pohanić, "Modeliranje biokatalitičke redukcije acetona katalizirane ketoreduktazom", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:149:522510

Metadata
TitleModeliranje biokatalitičke redukcije acetona katalizirane ketoreduktazom
Title (english)Modelling of biocatalytical acetone reduction catalyzed by ketoreductase
AuthorPetra Pohanić
MentorAna Vrsalović Presečki (mentor)
Committee memberAna Vrsalović Presečki (predsjednik povjerenstva)
Committee memberZvjezdana Findrik Blažević (član povjerenstva)
Committee memberAna Lončarić Božić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2018-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Reaction Engineering
Abstract
Ketoreduktaze su enzimi koji sudjeluju u prijenosu elektrona, vodikovih ili kisikovih atoma te time kataliziraju redoks reakcije. U ovom radu provedeno je ispitivanje kinetike ketoreduktaze (KRED) u reakciji redukcije acetona, kao i ispitivanje kinetike u povratnoj reakciji, oksidaciji izopropanola. KRED za redukciju acetona zahtijeva prisustvo koenzima nikotin-amid-adenin-dinukleotid-fostata (NADPH) koji dovodi vodik do acetona reducirajući ga u izopropanol, prilikom čega NADPH prelazi u oksidirani oblik, NADP+. Eksperimentalni podaci obrađeni su u programskom paketu Scientist, a kinetički parametri procijenjeni su nelinearnom regresijom pomoću simpleks metode ili metode najmanjih kvadrata. Također, određena je stabilnost enzima u otopini acetona. Na temelju sheme reakcije i procijenjenih kinetičkih parametara postavljen je matematički model. Upotrebom modela provedena je simulacija redukcije acetona katalizirane ketoreduktazom u kotlastom reaktoru. Zaključeno je kako se reakcija redukcije acetona katalizirana ketoreduktazom može koristiti u procesu regeneracije koenzima NADP+ zbog postignute ravnotežne konverzije od 84,7 %.
Abstract (english)
Ketoreductase is an enzyme involved in the transfer of electrons, hydrogen or oxygen atoms, thus catalyzing the redox reaction. In this paper, ketoreductase kinetics (KRED) was investigated in the reaction of acetone reduction as well as the kinetic in the reverse reaction, the isopropanol oxidation. KRED for acetone reduction requires the presence of nicotinamide-adenine-dinucleotide-phostate (NADPH) coenzyme, which transfers the hydrogen to acetone by converting it into isopropanol, whereby NADPH passes into an oxidized form, NADP+. Experimental data were processed in the Scientist program package, and kinetic parameters were estimated by nonlinear regression using the simplex method or the least squares method. Also, the stability of the enzyme in the acetone solution was determined. Based on reaction scheme and estimated kinetic parameters, a mathematical model was set. Using the model, the simulation of acetone reduction catalyzed by ketoreductase was done in the batch reactor. It was concluded that reaction of acetone reduction catalyzed by examine ketoreductase can be used in the NADP+ coenzyme regeneration process due to achieved 84,7 % equilibrium conversion.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:522510
ProjectNumber: 635595
Title: Sustainable industrial processes based on a C-C bond-forming enzyme platform
Acronym: CARBAZYMES
Leader: Zvjezdana Findrik Blažević
Jurisdiction: eu
Funder: EC
Funding stream: H2020
Study programmeTitle: Environmental Engineering - Undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2022-05-20 13:39:07