Biodiesel is a mixture of fatty acid alkyl esters and is most commonly produced from edible vegetable oils or animal fats. It is considered a clean, biodegradable and non-toxic alternative to diesel fuel. For its production and use to be sustainable, it needs to be synthesized from inedible waste feedstocks. This reduces the problem of waste disposal and avoids ethical conflicts associated with the use of food for energy purposes. Considering the large global production and consumption of coffee, its waste residue is considered a potential raw material for the production of biodiesel due to its high oil content and high availability worldwide. The topic of this paper is the production of biodiesel from waste coffee grounds. Oil was obtained from waste coffee grounds using the Soxhlet extraction method. It was purified by liquid-liquid extraction with a deep eutectic solvent in order to reduce the amount of free fatty acids in the oil itself. Biodiesel was produced from the extracted oil via the transesterification process with an alkaline catalyst. Crude biodiesel was then purified by liquid-liquid extraction, which removed all residual glycerol in the biodiesel. This paper explored the influence of reaction conditions on the process efficiency and the applicability of deep eutectic solvents in the process of biodiesel production from waste coffee grounds. Produced biodiesel was mixed with diesel fuel and the obtained mixture was physically and chemically characterized.