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undergraduate thesis
Razvoj i validacija kromatografske metode za određivanje sulfametoksazola u vodi
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2022. urn:nbn:hr:149:542596
Petko, Ramona
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Petko, R. (2022). Razvoj i validacija kromatografske metode za određivanje sulfametoksazola u vodi (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:542596

Petko, Ramona. "Razvoj i validacija kromatografske metode za određivanje sulfametoksazola u vodi." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2022. https://urn.nsk.hr/urn:nbn:hr:149:542596

Petko, Ramona. "Razvoj i validacija kromatografske metode za određivanje sulfametoksazola u vodi." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2022. https://urn.nsk.hr/urn:nbn:hr:149:542596

Petko, R. (2022). 'Razvoj i validacija kromatografske metode za određivanje sulfametoksazola u vodi', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 01 April 2025, https://urn.nsk.hr/urn:nbn:hr:149:542596

Petko R. Razvoj i validacija kromatografske metode za određivanje sulfametoksazola u vodi [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2022 [cited 2025 April 01] Available at: https://urn.nsk.hr/urn:nbn:hr:149:542596

R. Petko, "Razvoj i validacija kromatografske metode za određivanje sulfametoksazola u vodi", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:149:542596

Metadata
TitleRazvoj i validacija kromatografske metode za određivanje sulfametoksazola u vodi
Title (english)Development and validation of chromatographic method for the determination of sulfamethoxazole in water
AuthorRamona Petko
MentorDragana Mutavdžić Pavlović (mentor)
Committee memberDragana Mutavdžić Pavlović (predsjednik povjerenstva)
Committee memberSandra Babić (član povjerenstva)
Committee memberSvjetlana Krištafor (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2022-09-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Cilj ovoga rada jest validacija analitičke kromatografske metode određivanja farmaceutika sulfametoksazola u vodi na HPLC-DAD uređaju. Validacija prethodno razvijene metode važna je iz razloga što će osigurati potvrdu o pouzdanosti i kvaliteti metode koja će se u budućnosti moći sa sigurnošću primjenjivati pri analizi određenog uzorka na određenom uređaju. Analitički sustav u ovome radu validiran je na način da su ispitane izvedbene značajke metode, tj. validacijski parametri poput... More selektivnosti, linearnosti, osjetljivosti, granice detekcije, granice kvantifikacije, preciznosti, istinitosti, radnog područja te robusnosti. Na temelju rezultata zaključuje se ispravnost ispitivane analitičke metode. Prilikom određivanja navedenih kriterija, provedeno je kvantitativno određivanje antibiotika sulfametoksazola otopljenog u deioniziranoj i izvorskoj vodi. Svaki eksperiment proveden je dodatkom standardne otopine sulfametoksazola u obje vrste vode, kako bi se usporedili rezultati dobiveni za eksperimente provedene s deioniziranom vodom koju karakterizira visoka čistoća, s onima u izvorskoj vodi koja sadrži razne interferencije. Na osnovu rezultata istraživanja, dolazi se do zaključka kako je navedena metoda pouzdana i ispravna, ili, drugim riječima, validirana. Istraživanje u ovom radu poslužit će za daljnja ispitivanja analitičara koji će imati zadatak kvalitativno i kvantitativno odrediti farmaceutik sulfametoksazol u uzorcima vode tekućinskom kromatografijom visoke djelotvornosti uz detektor s nizom dioda. Less
Abstract (english)
The aim of this work is to validate the chromatographic analytical method for the determination of sulfamethoxazole in water on a HPLC-DAD instrument. Validation of a previously developed method is important because it provides confirmation of the reliability and quality of the method, which can be used with confidence in the future when analyzing a particular sample on a particular instrument. The analytical system in this work was validated by examining the method performance... More characteristics, i.e., validation parameters such as selectivity, linearity, sensitivity, limit of detection, limit of quantification limits, precision, trueness, working range, and robustness. Based on the results, the correctness of the investigated analytical method is concluded. To determine the above criteria, a quantitative determination of the antibiotic sulfamethoxazole dissolved in deionized and spring water was performed. Each experiment was carried out with samples in both types of water in order to compare the results of experiments with deionized water, which is characterized by high purity, with the results of experiments with spring water, which contains various interferences. Based on the research results, it is concluded that the mentioned method is reliable and correct, or, in other words, it is validated. The research in this work will serve for further tests of analysts who have the task to qualitatively and quantitatively determine the drug sulfamethoxazole in water samples by high-performance liquid chromatography with a diode array detector. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:542596
Study programmeTitle: Environmental Engineering - Undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-10-01
Terms of useLicence In copyright icon
Created on2023-03-13 09:21:31
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