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master's thesis
Biorazgradnja polistirenskih mikroplastičnih čestica bakterijskom kulturom Bacillus cereus
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2022. urn:nbn:hr:149:020860
Badurina-Petričević, Amadea
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Badurina Petričević, A. (2022). Biorazgradnja polistirenskih mikroplastičnih čestica bakterijskom kulturom Bacillus cereus (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:020860

Badurina Petričević, Amadea. "Biorazgradnja polistirenskih mikroplastičnih čestica bakterijskom kulturom Bacillus cereus." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2022. https://urn.nsk.hr/urn:nbn:hr:149:020860

Badurina Petričević, Amadea. "Biorazgradnja polistirenskih mikroplastičnih čestica bakterijskom kulturom Bacillus cereus." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2022. https://urn.nsk.hr/urn:nbn:hr:149:020860

Badurina Petričević, A. (2022). 'Biorazgradnja polistirenskih mikroplastičnih čestica bakterijskom kulturom Bacillus cereus', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 23 March 2025, https://urn.nsk.hr/urn:nbn:hr:149:020860

Badurina Petričević A. Biorazgradnja polistirenskih mikroplastičnih čestica bakterijskom kulturom Bacillus cereus [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2022 [cited 2025 March 23] Available at: https://urn.nsk.hr/urn:nbn:hr:149:020860

A. Badurina Petričević, "Biorazgradnja polistirenskih mikroplastičnih čestica bakterijskom kulturom Bacillus cereus", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:149:020860

Metadata
TitleBiorazgradnja polistirenskih mikroplastičnih čestica bakterijskom kulturom Bacillus cereus
Title (english)Biodegradation of polystyrene microplastic particles by bacterial culture of Bacillus cereus
AuthorAmadea Badurina-Petričević
MentorDajana Kučić Grgić (mentor)
Committee memberDajana Kučić Grgić (predsjednik povjerenstva)
Committee memberVesna Ocelić Bulatović (član povjerenstva)
Committee memberLidija Furač (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2022-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
''Tradicionalna'' plastika koju dobivamo iz ugljena, prirodnog plina i nafte jedna je od ekološki štetnijih tvari koje proizvodi čovjek, no ona je vrlo važna i koristna za izgradnju kvalitetnog životnog okruženja. Plastika je iznimno primjenjiv materijal; transparentan, isplativ, lagan, jak i izdržljiv, te posjeduje svojstva koja ga čine korisnima u medicini, poljoprivredi, domaćinstvu te u industrijskoj primjeni. Iako su navedena svojstva poželjna, vrlo stabilna struktura plastike otežava... More proces biorazgradnje. Plastični materijali se prekomjerno koriste, neodgovorno odbacuju te kao takvi opstaju u tlu, moru i slatkovodnim ekosustavima te se razlažu na manje čestice. Sve plastične čestice manje od 5 mm nazivaju se mikroplastikom (MP). Kroz godine istraživanja, utvrđeni su štetni učinci aditiva koji se dodaju plastici tijekom procesa proizvodnje. Stoga je od izuzetne važnosti pronaći učinkovitu metodu uklanjanja mikroplastike iz okoliša. Sadašnje znanje o aerobnoj biorazgradnji ističe potencijal mikrobnih kultura za uklanjenje mikroplastike iz okoliša. U ovom radu ispitala se biorazgradnja polistirena (PS) primjenom bakterijske kulture Bacillus cereus pri određenim optimalnim uvjetima. Plan eksperimenta biorazgradnje dizajniran je prema punom faktorskom planu. Na temelju preliminarnih pokusa odredili su se značajni čimbenici za biorazgradnju PS-a. Tijekom procesa pratio se broj izraslih stanica bakterija (CFU), koncentracija ukupnog organskog ugljika (TOC), ukupnog ugljika (TC) i anorganskog ugljika (IC) te je provedena LC/MS analiza u svrhu identificiranja otpuštenih aditiva s površine MP-a ili nastalih razgradnih organskih produkata. Uzorci su okarakterizirani FTIR-ATR analizom. Na kraju svih procesa biorazgradnje ispitana je i ekotoksičnost filtrata primjenom bakterije Vibrio fischeri radi utvrđivanja postojećih potencijalnih štetnih učinaka na ispitivani organizam. Eksperimentalni rezultati obrađeni su korištenjem statističke analize varijance (ANOVA). Rezultati pokusa ukazuju da je bolji rast bakterije Bacillus cereus uočen pri najmanjoj veličini čestica (<300 μm), najmanjoj vrijednosti koncentracije (50 mg/L) i najnižem broju okretaja (100 o/min). S obzirom na navedene optimalne uvjete, ispitivana bakterijska kultura ima sposobnost biorazgradnje MP-a. Less
Abstract (english)
"Conventional" plastic, derived from coal, natural gas and petroleum, is one of the most polluting substances produced by man, but it is very important and useful for building a livable environment. Plastic is extremely versatile, transparent, inexpensive, lightweight, strong and durable, and has properties that make it useful in medicine, agriculture, household and industry. Although these properties are desirable, a very stable plastic structure makes biodegradation difficult. Plastics... More are overused, irresponsibly discarded, and thus survive in soil, marine, and freshwater ecosystems where they decompose into smaller particles. All plastic particles smaller than 5 mm are called microplastics. Over the years, research has identified the harmful effects of additives added to plastics during the production process. Therefore, it is extremely important to find an effective method to remove microplastics from the environment. Current knowledge on biotechnological aerobic biodegradation shows the potential of microbial cultures to remove microplastics from the environment. In this work, the biodegradation of polystyrene (PS) was studied using a bacterial culture of Bacillus cereus under certain optimal conditions. The experimental design for biodegradation was designed according to the Full Factorial Plan. Based on preliminary experiments, important factors for the biodegradation of PS were determined. During the experiments, the colony forming number (CFU), the concentration of total organic carbon (TOC), total carbon (TC) and inorganic carbon (IC) were monitored, and LC / MS analysis was performed to identify the additives released from the surface of MP and the resulting organic degradation products. Samples were characterized by FTIR-ATR analysis. At the end of all biodegradation processes, the ecotoxicity of the filtrate was tested using Vibrio fischeri to determine the potential adverse effects present on the test organism. The experimental results were evaluated using statistical analysis of variance (ANOVA). The experimental results show that better growth of the bacterium Bacillus cereus was observed at the smallest particle size (< 300 μm), the smallest concentration value (50 mg/L) and the lowest rotation speed (100 rpm). Under the mentioned optimal conditions, the tested bacterial culture was able to biodegrade MPs. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:020860
ProjectNumber: IP-2019-04-9661
Title: Primjena naprednih tehnologija obrade voda za uklanjanje mikroplastike
Title: Advanced Water Treatment Technologies for Microplastics Removal
Acronym: AdWaTMiR
Leader: Tomislav Bolanča
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Environmental Engineering - Graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2024-06-01
Terms of useLicence In copyright icon
Created on2023-03-23 12:56:32
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