prikaz prve stranice dokumenta Imobilizacija enzima na mezoporoznu siliku funkcionalizarnu (3-aminopropil)trietoksisilanom
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master's thesis
Imobilizacija enzima na mezoporoznu siliku funkcionalizarnu (3-aminopropil)trietoksisilanom
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2022. urn:nbn:hr:149:947977
Markanović, Laura
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Markanović, L. (2022). Imobilizacija enzima na mezoporoznu siliku funkcionalizarnu (3-aminopropil)trietoksisilanom (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:947977

Markanović, Laura. "Imobilizacija enzima na mezoporoznu siliku funkcionalizarnu (3-aminopropil)trietoksisilanom." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2022. https://urn.nsk.hr/urn:nbn:hr:149:947977

Markanović, Laura. "Imobilizacija enzima na mezoporoznu siliku funkcionalizarnu (3-aminopropil)trietoksisilanom." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2022. https://urn.nsk.hr/urn:nbn:hr:149:947977

Markanović, L. (2022). 'Imobilizacija enzima na mezoporoznu siliku funkcionalizarnu (3-aminopropil)trietoksisilanom', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:149:947977

Markanović L. Imobilizacija enzima na mezoporoznu siliku funkcionalizarnu (3-aminopropil)trietoksisilanom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2022 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:149:947977

L. Markanović, "Imobilizacija enzima na mezoporoznu siliku funkcionalizarnu (3-aminopropil)trietoksisilanom", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:149:947977

Metadata
TitleImobilizacija enzima na mezoporoznu siliku funkcionalizarnu (3-aminopropil)trietoksisilanom
Title (english)Enzyme immobilisation on mesoporous silica functionalised by (3-aminopropyl)triethoxysilane
AuthorLaura Markanović
MentorAna Vrsalović Presečki (mentor)
Committee memberAna Vrsalović Presečki (predsjednik povjerenstva)
Committee memberGordana Matijašić (član povjerenstva)
Committee memberMarija Vuković Domanovac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2022-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
2-Deoksi-D-riboza-5-fosfat aldolaza (DERA) je katalizator u reakciji dvostruke aldolne adicije acetaldehida i kloroacetaldehida gdje kao produkt nastaje 6-kloro-3,5-dihidroksiheksanal (laktol), intermedijer za proizvodnju statina, lijekova za snižavanje kolesterola. Međutim, visoka koncentracija aldehida ima vrlo negativan utjecaj na stabilnost enzima u reakciji stoga je cilj ovog rada provesti imobilizaciju enzima DERA-e kovalentnim vezanjem na MCF siliku kao nosioc, u svrhu njegove stabilizacije. Silika je sintetizirana sol-gel metodom te je prethodno imobilizaciji funkcionalizirana APTES-om, potom aktivirana. Provedeno je optimiranje vrste i koncentracije aktivacijskog sredstva te su u tu svrhu ispitivani su benzokinon, glutaraldehid te GPTMS različitih koncentracija. Potom je također ispitan utjecaj pH vrijednosti imobilizacijskog pufera na uspješnost imobilizacije. Pokazalo se da su optimalni uvjeti imobilizacije dobiveni s 15%-tnim glutaraldehidom pri pH 6. Konačno, optimalni uvjeti su ispitani na silici većih pora te je pokazano kako promjer pora ima utjecaj na uspješnost imobilizacije.
Abstract (english)
2-Deoxy-D-ribose-5-phosphate aldolase (DERA) is a catalyst in the double aldol addition reaction of acetaldehyde and chloroacetaldehyde, where 6-chloro-3,5-dihydroxyhexanal (lactol) is formed as a product, an intermediate in the production of statins, drugs used to lower cholesterol. However, a high concentration of the aldehyde has a negative effect on the stability of the enzyme in the reaction. The aim of this work is to immobilize the enzyme DERA by covalent binding to MCF silica as a carrier, in order to stabilize it. The silica was synthesized by sol-gel method and functionalized with APTES and then activated before immobilization. Optimization of the type and concentration of the activating agent was performed, and for this purpose, benzoquinone, glutaraldehyde and GPTMS were tested at different concentrations. Subsequently, the influence of the pH of the immobilization buffer on the success of immobilization was also investigated. It was found that the optimal immobilization conditions were obtained with 15% glutaraldehyde at pH 6. Finally, the optimal conditions were tested on silica with larger pores, and it was shown that the pore diameter has an influence on the success of immobilization.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:947977
Study programmeTitle: Materials Science and Engineering - Graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
Type of resourceText
File originBorn digital
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Created on2023-03-28 10:20:07