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master's thesis
Sinteza cijanoalkohola katalizirana halogenhidrin-dehalogenazom
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2021. urn:nbn:hr:149:352899
Mateša, Lorena
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

Cite this document

Mateša, L. (2021). Sinteza cijanoalkohola katalizirana halogenhidrin-dehalogenazom (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:352899

Mateša, Lorena. "Sinteza cijanoalkohola katalizirana halogenhidrin-dehalogenazom." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2021. https://urn.nsk.hr/urn:nbn:hr:149:352899

Mateša, Lorena. "Sinteza cijanoalkohola katalizirana halogenhidrin-dehalogenazom." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2021. https://urn.nsk.hr/urn:nbn:hr:149:352899

Mateša, L. (2021). 'Sinteza cijanoalkohola katalizirana halogenhidrin-dehalogenazom', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:149:352899

Mateša L. Sinteza cijanoalkohola katalizirana halogenhidrin-dehalogenazom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2021 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:149:352899

L. Mateša, "Sinteza cijanoalkohola katalizirana halogenhidrin-dehalogenazom", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:149:352899

Metadata
TitleSinteza cijanoalkohola katalizirana halogenhidrin-dehalogenazom
Title (english)Synthesis of cyano-alcohol catalysed by halohydrin dehalogenase
AuthorLorena Mateša
MentorZvjezdana Findrik Blažević (mentor)
Committee memberZvjezdana Findrik Blažević (predsjednik povjerenstva)
Committee memberMartina Sudar (član povjerenstva)
Committee memberMarija Vuković Domanovac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2021-06-21, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
Enzimi su biokatalizatori prisutni u mikroorganizmima, a djeluju poput anorganskih katalizatora. Njihova uporaba osigurava niz prednosti, poput odvijanja reakcija u blažim uvjetima te smanjenje nastanka štetnih nusprodukata. Halogenhidrin-dehalogenaze su enzimi koji kataliziraju reakciju dehalogenacije haloalkohola uz stvaranje epoksida, kao i reverzibilnu reakciju, reakciju otvaranja epoksidnog prstena s nukleofilima. U ovom je radu ispitana reakcija otvaranja epoksidnog prstena 2-(4-trifluormetil)fenil oksirana, kataliziranu C tipom halogenhidrin-dehalogenaze HheC-W249P uz natrijev cijanid kao nukleofil, u kotlastom reaktoru i mikroreaktoru. Reakcija je praćena uporabom HPLC analize, a aktivnost enzima je praćena spektrofotometrijski. Osim reakcije otvaranja epoksidnog prstena, istraživane su i reakcija hidrolize epoksida i kemijska reakcija otvaranja epoksidnog prstena. Reakciju je moguće provesti i u kotlastom reaktoru i u mikroreaktoru. U kotlastom reaktoru količina nastalog produkta se povećava s vremenom odvijanja reakcije. U mikroreaktoru, najviše produkta nastane pri najvećem vremenu zadržavanja, ali tada se epoksid troši i sporednim reakcijama. Brzina deaktivacije enzima se povećava porastom prostornog vremena zadržavanja.
Abstract (english)
Enzymes are biocatalysts present in microorganisms and act like inorganic catalysts. Their usage provides a number of benefits, such as reactions in milder conditions and a reduction in occurence of harmful by.products. Halohydrin dehalogenases are enzymes that catalyze dehalogenation od haloalcohols to form epoxides, as well as the reversible reaction, the ring-opening reaction with nucleophiles. In this work, the ring-opening reaction of 2-(4-trifluormethyl)phenyl oxirane with sodium cyanide as nucleophile, catalyzed by C-type halohydrin dehalogenase HheC-W249P, in batch reactor and microreactor,was investigated. The reaction was monitored using HPLC analysis and the enzyme activity was monitored spectrofotometrically. In addition to epoxy ring-opening reaction, the hydrolysis reaction of epoxide and the chemical ring opening reaction of epoxide were also tested. The reaction can be carried out in both the batch reactor and the microreactor. In the batch reactor, the amount of the product formed increases with the reaction time. In the microreactor, the highest amount of product formed was observed at highest retention time, but then the epoxide is consumed in side reactions. Also, enzyme deactivation rate increases with increase of retention times in the microreactor.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:352899
ProjectNumber: IP-2018-01-4493
Title: Enzimska sinteza fluoriranih kiralnih građevnih blokova
Acronym: EnzyFluor
Leader: Maja Majerić-Elenkov
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Chemical Engineering - Graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-10-31
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Created on2023-04-05 13:30:16