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master's thesis
Matematički model biokatalitičke sinteze nanočestica
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2015. urn:nbn:hr:149:759585
Jovičić, Mirna
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

Cite this document

Jovičić, M. (2015). Matematički model biokatalitičke sinteze nanočestica (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:759585

Jovičić, Mirna. "Matematički model biokatalitičke sinteze nanočestica." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2015. https://urn.nsk.hr/urn:nbn:hr:149:759585

Jovičić, Mirna. "Matematički model biokatalitičke sinteze nanočestica." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2015. https://urn.nsk.hr/urn:nbn:hr:149:759585

Jovičić, M. (2015). 'Matematički model biokatalitičke sinteze nanočestica', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:149:759585

Jovičić M. Matematički model biokatalitičke sinteze nanočestica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2015 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:149:759585

M. Jovičić, "Matematički model biokatalitičke sinteze nanočestica", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:149:759585

Metadata
TitleMatematički model biokatalitičke sinteze nanočestica
Title (english)Mathematical modelling of biocatalytical synthesis of nanoparticles
AuthorMirna Jovičić
MentorAna Vrsalović Presečki (mentor)
Committee memberAna Vrsalović Presečki (predsjednik povjerenstva)
Committee memberZvjezdana Findrik Blažević (član povjerenstva)
Committee memberFabio Faraguna (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2015-07-13, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Reaction Engineering
Abstract
U ovom radu provedena je sinteza nanočestica kalcijevog karbonata pomoću produkata biokatalitičke hidrolize uree i enzima ureaze. Uz to ispitivana je kinetika hidrolize uree uz enzim ureazu te je promatran utjecaj kalcijevih (Ca2+) iona na aktivnost enzima. Reakcije su provedene u dva medija – redestiliranoj vodi i 10%-tnoj otopini Tritona x-100, pri dvije temperature, T = 30°C i T = 20°C. Ispitivanje kinetike reakcije provedeno je mjerenjem početne brzine reakcije pri različitim koncentracijama uree, uz stalnu koncentraciju enzima ureaze. Kinetika reakcije opisana je jednosupstratnom Michaelis – Menteničinom kinetikom. Utjecaj kalcijevih iona (Ca2+) na aktivnost enzima ispitan je mjerenjem specifične aktivnosti ureaze u ovisnosti o različitim koncentracijama kalcijeva klorida, CaCl2, uz stalnu koncentraciju uree i ureaze. Utvrđeno je da kalcijevi ioni inhibiraju ureazu nekompetitivnom inhibicijom. Razvijen je matematički model procesa koji je validiran provođenjem reakcije u kotlastom reaktoru uz stalno miješanje na termostatiraoj tresilici. Reakcijom kalcijevih iona s produktima hidrolize uree istaložene su nanočestice kalcijevog karbonata koje su karakterizirane pomoću pretražnog elektronskog mikroskopa.
Abstract (english)
In this study synthesis of calcium carbonate nanoparticles was carried out using products of biocatalytical hydrolysis of urea catalyzed by enzyme urease. Kinetics of the urease-catalyzed hydrolysis of urea and the influence of Ca2+ ions on enzyme activity was tested. Reactions were carried out in two different media – redistilled water and 10% Triton x-100 solution at two temperatures, T = 30°C and T = 20°C. Reaction kinetics was experimentally determined using the initial reaction rate method. Reaction kinetics was described with Michaelis – Menten kinetic model. Influence of calcium ions (Ca2+) on the enzyme activity was tested by measuring the specific activity of urease as a function of various concentrations of calcium chloride, CaCl2, with a constant concentration of urea and urease. It was found that Ca2+ ions inhibit urease with non – competitive inhibition. Mathematical model of the process was developed and validated in a batch reactor. Nanoparticle synthesis was carried out by adding calcium chloride into a solution of urea and enzyme urease. In the reaction of calcium ions and urea hydrolysis products, calcium carbonate nanoparticles were precipitated. Synthesized nanoparticles were characterized with scanning electron microscope.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:759585
Study programmeTitle: Environmental Engineering - Graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2016-12-16 14:01:17