Enzymes are biological catalysts that are present in living cells and are necessary for life as we know it. Reactions in which enzymes are present need to be well investigated and characterized in order to be carried out on an industrial scale. The advantage of using enzymes in biocatalytic reactions lies in the fact that they are effective even under very mild conditions, much milder than catalysts of chemical reactions. It is necessary to pay attention to the activity of enzymes in different reaction conditions (temperature, substrate concentration, pH, oxygen saturation) because by optimizing the reaction conditions we enable maximum yield of the desired product and reduction of unwanted or harmful by-products. Fluorinated aromatic compounds are extremely important in the pharmaceutical industry as precursors and as building blocks. It is important that such compounds be optically pure because it is known that the two enantiomers can have significantly different properties and biological effect, often harmful and toxic. One of the methods of synthesis of such compounds is the use of the enzyme halohydrin-dehalogenase (HHDH). In this paper, the reaction of opening an epoxy ring, a fluorinated derivative of styrene oxide with an azide as a nucleophile, during which a fluorinated azidoalcohol is formed, was studied. The reaction is catalyzed by the HHDH (C-type) enzyme also known as HheC-W249P. The aim of this work was to validate a mathematical model of biocatalytic synthesis of (R)-2-azido-1-(4-trifluoromethyl-phenyl)-ethanol in a batch reactor. The model was successfully validated by carrying out the reaction in five different batch reactors with different initial conditions in the reactor. Samples of the reaction mixture were analyzed on a high performance liquid chromatograph. The stability of the enzyme under different conditions was also monitored, and it was shown that the initial conditions in the reactor did not significantly affect the stability of the enzyme. Enzyme stability was monitored by spectrophotometric method.