Plants are an important part of human nutrition and have been used for therapeutic purposes since ancient times. Their secondary metabolites, polyphenols, in addition to playing an important role in plant physiology, show a number of health benefits that are increasingly being explored. Due to the beneficial components they possess, there is an increase in the use of plants in the food, cosmetics and pharmaceutical industries. In this paper, the extracted polyphenol concentration from the crushed and dried plantain leaves was investigated using a deep eutectic solvent. Deep eutectic solvents are taking part in green chemistry and are finding an increasing use in chemical processes. Three deep eutectic solvents that were used in this paper were prepared from the components betaine, fructose and glycerol, lactic acid and glycerol, and lactic acid, fructose and glycerol. Extraction was performed on a magnetic stirrer and the viscosity and polarity test of the prepared solvents was performed. For further extraction testing, a solvent with lactic acid, fructose and glycerol was selected which showed the highest polarity and the lowest viscosity. Extractions were performed with magnetic stirrer, shaker, ultrasonic probe and ultrasonic bath at different times. The highest concentration of polyphenols is obtained by extraction on a magnetic stirrer, and the best time of the proven process is achieved by extraction using ultrasonic probes.