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undergraduate thesis
Pročišćavanje enzima MenD za primjenu u biokatalizi
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2021. urn:nbn:hr:149:655843
Todorović, Nolla
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

Cite this document

Todorović, N. (2021). Pročišćavanje enzima MenD za primjenu u biokatalizi (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:655843

Todorović, Nolla. "Pročišćavanje enzima MenD za primjenu u biokatalizi." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2021. https://urn.nsk.hr/urn:nbn:hr:149:655843

Todorović, Nolla. "Pročišćavanje enzima MenD za primjenu u biokatalizi." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2021. https://urn.nsk.hr/urn:nbn:hr:149:655843

Todorović, N. (2021). 'Pročišćavanje enzima MenD za primjenu u biokatalizi', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 27 November 2024, https://urn.nsk.hr/urn:nbn:hr:149:655843

Todorović N. Pročišćavanje enzima MenD za primjenu u biokatalizi [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2021 [cited 2024 November 27] Available at: https://urn.nsk.hr/urn:nbn:hr:149:655843

N. Todorović, "Pročišćavanje enzima MenD za primjenu u biokatalizi", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:149:655843

Metadata
TitlePročišćavanje enzima MenD za primjenu u biokatalizi
Title (english)Purification of enzyme MenD for application in biocatalysis
AuthorNolla Todorović
MentorMartina Sudar (mentor)
Committee memberAnita Šalić (predsjednik povjerenstva)
Committee memberDajana Kučić Grgić (član povjerenstva)
Committee memberAna Vrsalović Presečki (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2021-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
Razvojem biotehnologije dolazi do novih otkrića u području biokatalize čija je najveća prednost nastajanje manje štetnih nusprodukata u odnosu na kemijsku katalizu. U biokatalizi se koriste različiti oblici biokatalizatora: pročišćeni i nepročišćeni enzimi kao i cijele stanice mikroorganizma. U ovom radu proveden je postupak pročišćavanja enzima MenD (sintaza 2-sukcinil-5-enolpiruvil-6-hidroksi-3-cikloheksen-1-karboksilne kiseline) s manjom (50 mg) i većom masom uzorka (200 mg). U svrhu pročišćavanja enzima korištena je metoda afinitetne kromatografije. Nakon pročišćavanja uzoraka afinitetnom kromatografijom korištena je SDS-PAGE elektroforeza za provjeru prisutnosti proteina u sakupljenim frakcijama te Bradfordova metoda za određivanje koncentracija proteina u frakcijama.
Abstract (english)
With the development of biotechnology, new discoveries are being made in the field of biocatalysis, the biggest advantage of which is the formation of less harmful by-products compared to chemical catalysis. Different forms of biocatalysts can be used: purified and crude enzyme as well as whole cells of the microorganisms. In this work, purification of the enzyme MenD (2-succinyl-5-enolpyruvil-6-hydroxy-3-cyclohexene-1-carboxylic acid synthase) with a lower mass (50 mg) and a higher mass sample (200 mg) was performed. Affinity chromatography was used to purify the sample. After purification of the samples by affinity chromatography, SDS-PAGE electrophoresis was used to prove the presence of proteins in the collected fractions and the Bradford method to determine protein concentration in the samples.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:655843
Study programmeTitle: Environmental Engineering - Undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2023-04-24 12:11:36