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undergraduate thesis
Validacija HPLC-PDA metode za određivanje ciprofloksacina u vodi
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2021. urn:nbn:hr:149:717503
Vuković, Martina
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

Cite this document

Vuković, M. (2021). Validacija HPLC-PDA metode za određivanje ciprofloksacina u vodi (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:717503

Vuković, Martina. "Validacija HPLC-PDA metode za određivanje ciprofloksacina u vodi." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2021. https://urn.nsk.hr/urn:nbn:hr:149:717503

Vuković, Martina. "Validacija HPLC-PDA metode za određivanje ciprofloksacina u vodi." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2021. https://urn.nsk.hr/urn:nbn:hr:149:717503

Vuković, M. (2021). 'Validacija HPLC-PDA metode za određivanje ciprofloksacina u vodi', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:149:717503

Vuković M. Validacija HPLC-PDA metode za određivanje ciprofloksacina u vodi [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2021 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:149:717503

M. Vuković, "Validacija HPLC-PDA metode za određivanje ciprofloksacina u vodi", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:149:717503

Metadata
TitleValidacija HPLC-PDA metode za određivanje ciprofloksacina u vodi
Title (english)HPLC-PDA method validation for the determination of ciprofloxacin in water
AuthorMartina Vuković
MentorSandra Babić (mentor)
Committee memberSandra Babić (predsjednik povjerenstva)
Committee memberIrena Škorić (član povjerenstva)
Committee memberMartina Biošić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2021-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Nakon razvitka pojedine metode prvi slijedeći korak jest validacija iste. Validacija analitičke metode definira se kao postupak dokazivanja odnosno utvrđivanja je li metoda prikladna za uporabu u željenu svrhu. Procesu validacije metode pristupa se laboratorijskim ispitivanjima pri čemu je nužno ispitivanje izvedbenih karakteristika: selektivnosti/specifičnosti metode, linearnosti, radnoga područja, preciznosti, istinitosti, granica kvantifikacije i detekcije te robusnosti. Primarni cilj ovoga rada bio je validirati unaprijed poznatu HPLC-PDA analitičku metodu za određivanje fluorokinolonskog antibiotika ciprofloksacina. Istraživanje je provedeno u svrhu upoznavanja cjelokupnog postupka validacije analitičke metode te donošenja adekvatnih zaključaka o prihvatljivosti iste. Određene su izvedbene karakteristike metode tekućinske kromatografije visoke djelotvornosti pri čemu su same otopine ciprofloksacina pripravljane u MilliQ i riječnoj vodi. Na temelju dobivenih rezultata provedenog istraživanja potvrđena je prihvatljivost metode za određivanje ciprofloksacina u vodi, s obzirom da se dobivene vrijednosti izvedbenih karakteristika nalaze unutar definiranih granica prihvatljivosti.
Abstract (english)
After the development of a particular method, the first next step is to validate it. Validation of an analytical method is defined as the process of proving or determining whether a method is suitable for use for the desired purpose. The method validation process is approached by laboratory tests, where it is necessary to examine the performance characteristics: selectivity/specificity of the method, linearity, working range, precision, trueness, limits of quantification and detection and also robustness. The primary goal of this work was to validate an already known HPLC-PDA analytical method for the determination of fluoroquinolone antibiotic ciprofloxacin. The research was conducted to familiarize with the entire process of validation of the analytical method and to make adequate conclusions about its acceptability. Performance characteristics of the high performance liquid chromatography method with a diode array detector are determined using ciprofloxacin solutions prepared in MiliQ and river water. Based on the obtained results of the conducted research, the applicability of the method for the determination of ciprofloxacin in water was confirmed, since the obtained values of performance characteristics meet all acceptability criteria.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:717503
Study programmeTitle: Materials Science and Engineering - Undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2022-09-01
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Created on2023-04-27 08:05:40