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undergraduate thesis
Validacija kromatografske metode za određivanje metoklopramida
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2020. urn:nbn:hr:149:691856
Vučemilović Šimunović, Luka
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Vučemilović Šimunović, L. (2020). Validacija kromatografske metode za određivanje metoklopramida (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:691856

Vučemilović Šimunović, Luka. "Validacija kromatografske metode za određivanje metoklopramida." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2020. https://urn.nsk.hr/urn:nbn:hr:149:691856

Vučemilović Šimunović, Luka. "Validacija kromatografske metode za određivanje metoklopramida." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2020. https://urn.nsk.hr/urn:nbn:hr:149:691856

Vučemilović Šimunović, L. (2020). 'Validacija kromatografske metode za određivanje metoklopramida', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 20 December 2024, https://urn.nsk.hr/urn:nbn:hr:149:691856

Vučemilović Šimunović L. Validacija kromatografske metode za određivanje metoklopramida [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2020 [cited 2024 December 20] Available at: https://urn.nsk.hr/urn:nbn:hr:149:691856

L. Vučemilović Šimunović, "Validacija kromatografske metode za određivanje metoklopramida", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:149:691856

Metadata
TitleValidacija kromatografske metode za određivanje metoklopramida
Title (english)Validation of chromatographic method for the determination of metoclopramide
AuthorLuka Vučemilović Šimunović
MentorSandra Babić (mentor)
Committee memberSandra Babić (predsjednik povjerenstva)
Committee memberDragana Vuk (član povjerenstva)
Committee memberDario Dabić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2020-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Validacija analitičke metode je nužan korak u osiguravanju kvalitete u analitičkoj kemiji. To je postupak koji obuhvaća definiranje zahtjeva, eksperimentalno ispitivanje izvedbenih karakteristika metode te davanje izjave o zadovoljavanju postavljenih zahtjeva. Kako bi se u potpunosti validirao sustav moraju se ispitati sve izvedbene karakteristike metode, a one su: specifičnost/selektivnost, linearnost, točnost, istinitost, preciznost, granica detekcije i kvantifikacije, radno područje i robusnost. Cilj ovog rada bio je validirati metodu za određivanje metoklopramida te ispitati karakteristike i na temelju rezultata zaključiti zadovoljava li analitička metoda kriterije prihvatljivosti. Analiza je provedena HPLC-MS/MS metodom na otopinama metoklopramida u MilliQ, izvorskoj i morskoj vodi, te su ispitane sve izvedbene karakteristike metode. Rezultati istraživanja su pokazali kako je metoda zadovoljila sve kriterije prihvatljivosti osim kriterija istinitosti za otopinu morske vode najmanje koncentracije. Iz toga možemo zaključiti kako je metoda prihvatljiva za određivanje metoklopramida.
Abstract (english)
Validation of the analytical method is a necessary step in ensuring quality in analytical chemistry. It is a procedure that includes defining requirements, experimental testing of method performance characteristics and giving a statement on meeting those requirements. In order to fully validate the system, all performance characteristics of the method must be examined, and they are: specificity/selectivity, linearity, accuracy, trueness, precision, limit of detection and quantitation, working range and ruggedness. The aim of this study was to validate the method for determination of metoclopramide and to examine the characteristics and, based on the results to conclude whether the analytical method meets the acceptance criteria. The analysis was performed by HPLC-MS/MS method on solutions of metoclopramide in MilliQ, spring and sea water and all performance characteristics of the method were examined. The result of the study showed that the method met all the acceptance criteria except the trueness criteria for the sea water solution of the lowest concentration. From this we can conclude that the method is acceptable for the determination of metoclopramide.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:691856
Study programmeTitle: Materials Science and Engineering - Undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2021-10-01
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Created on2023-05-09 10:27:19