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master's thesis
Ekstrakcija polifenolnih spojeva iz kožice grožđa raspršenjem matice uzorka kroz čvrstu fazu
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2015. urn:nbn:hr:149:040538
Prišlin, Barbara
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Prišlin, B. (2015). Ekstrakcija polifenolnih spojeva iz kožice grožđa raspršenjem matice uzorka kroz čvrstu fazu (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:040538

Prišlin, Barbara. "Ekstrakcija polifenolnih spojeva iz kožice grožđa raspršenjem matice uzorka kroz čvrstu fazu." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2015. https://urn.nsk.hr/urn:nbn:hr:149:040538

Prišlin, Barbara. "Ekstrakcija polifenolnih spojeva iz kožice grožđa raspršenjem matice uzorka kroz čvrstu fazu." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2015. https://urn.nsk.hr/urn:nbn:hr:149:040538

Prišlin, B. (2015). 'Ekstrakcija polifenolnih spojeva iz kožice grožđa raspršenjem matice uzorka kroz čvrstu fazu', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 02 June 2024, https://urn.nsk.hr/urn:nbn:hr:149:040538

Prišlin B. Ekstrakcija polifenolnih spojeva iz kožice grožđa raspršenjem matice uzorka kroz čvrstu fazu [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2015 [cited 2024 June 02] Available at: https://urn.nsk.hr/urn:nbn:hr:149:040538

B. Prišlin, "Ekstrakcija polifenolnih spojeva iz kožice grožđa raspršenjem matice uzorka kroz čvrstu fazu", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:149:040538

Metadata
TitleEkstrakcija polifenolnih spojeva iz kožice grožđa raspršenjem matice uzorka kroz čvrstu fazu
Title (english)Extraction of polyphenolic compounds from grape skin by matrix solid-phase dispersion
AuthorBarbara Prišlin
MentorDanijela Ašperger (mentor)
Committee memberDanijela Ašperger (predsjednik povjerenstva)
Committee memberTatjana Gazivoda Kraljević (član povjerenstva)
Committee memberJasminka Karoglan Kontić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2015-09-22, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Analytic Chemistry
Abstract
Polifenoli predstavljaju skupinu spojeva biljnog podrijetla, čiju strukturu čini jedan ili više aromatskih prstena s jednom ili više hidroksilnih skupina. Uobičajena su sastavnica ljudske prehrane te se u različitim količinama nalaze u voću, povrću, vinu, voćnim sokovima, čaju i kavi. Polifenoli se u crnom grožđu nalaze u kožici, sjemenki i mesu. Sastav i količina pojedinih polifenola ovisi o sorti grožđa, klimatskim uvjetima, postupcima uzgoja vinove loze. Cilj ovog rada je optimizacija uvjeta za ekstrakciju polifenola iz kožice grožđa metodom raspršenjem matice uzorka kroz čvrstu fazu (MSPD). Cilj optimizacije metode je pronaći optimalne čimbenike za ekstrakciju, a to su udio organske faze, volumen ekstrakcijskog otapala, omjer mase sorbensa i mase uzorka, optimalni sorbens i ekstracijsko otapalo. Ekstrakti su analizirani tekućinskom kromatografijom visoke djelotvornosti s detektorom s nizom dioda i fluorescentnim detektorom. Najbolji rezultat za ekstrakciju polifenola iz kožice grožđa dobiven je s fenilsilicijevim dioksidom kao MSPD sorbensom s 10 mL smjese otapala acetonitril:voda=50:50 kao ekstrakcijskim otapalom. Najbolji omjer mase uzorak/sorbens pokazao se omjer 125 mg uzorka/375 mg sorbensa.
Abstract (english)
Polyphenols are organic compounds, which structure makes one or more aromatic rings with one or more hydroxyl groups. Polyphenols colud be find in food especially fruits, vegetables, wine, fruit juices, tea and coffee. In red grapes we can find polyphenols in grape skin, seeds and pulp. The composition and quantity of particular polyphenols depends on grape cultivar, climatic conditions, methods of grapevine growing. The aim of this work is optimization conditions for extraction of polyphenolic compounds from grape skin by matrix solid-phase dispersion (MSPD). Parameters which were optimized are percent of organic eluation solvent, volume of eluation solvent, ratio of mass sample/sorbent, sorbent and the eluation solvent. The extracts were analyzed by high-performance liquid chromatography with diode array detector (HPLC-DAD) and fluorescence detector (HPLC-FLD). The best results for extraction of polyphenolic compounds from grape skin are obtained with Phenyl as an MSPD sorbent with 10 mL of acetonitrile:water=50:50 as an elution solvent. The best ratio of mass sample/sorbent is 125 mg sample/375 mg sorbent.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:040538
Study programmeTitle: Applied Chemistry - Graduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry
Course: Environmental chemistry and green technologies
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra primijenjene kemije (magistar/magistra primijenjene kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-03-06 13:55:00