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undergraduate thesis
Kinetička karakterizacija ketoreduktaze
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2019. urn:nbn:hr:149:718130
Pakšec, Vanda
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Pakšec, V. (2019). Kinetička karakterizacija ketoreduktaze (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:718130

Pakšec, Vanda. "Kinetička karakterizacija ketoreduktaze." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2019. https://urn.nsk.hr/urn:nbn:hr:149:718130

Pakšec, Vanda. "Kinetička karakterizacija ketoreduktaze." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2019. https://urn.nsk.hr/urn:nbn:hr:149:718130

Pakšec, V. (2019). 'Kinetička karakterizacija ketoreduktaze', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 09 November 2024, https://urn.nsk.hr/urn:nbn:hr:149:718130

Pakšec V. Kinetička karakterizacija ketoreduktaze [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2019 [cited 2024 November 09] Available at: https://urn.nsk.hr/urn:nbn:hr:149:718130

V. Pakšec, "Kinetička karakterizacija ketoreduktaze", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:149:718130

Metadata
TitleKinetička karakterizacija ketoreduktaze
Title (english)Kinetic characterization of the ketoreductase
AuthorVanda Pakšec
MentorAna Vrsalović Presečki (mentor)
Committee memberAna Vrsalović Presečki (predsjednik povjerenstva)
Committee memberVanja Kosar (član povjerenstva)
Committee memberDajana Kučić Grgić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2019-07-12, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
U ovom radu ispitivana je kinetika reakcije redukcije propanala uz biokatalizator ketoreduktazu (KRED), koji za svoju aktivnost zahtijeva prisustvo koenzima nikotin-amid-adenin-dinukleotid-fostata (NADPH). Kinetika je određena metodom početnih brzina reakcije, mjerenjem ovisnosti brzine reakcije o koncentraciji supstrata (propanal, NADPH). Također ispitao se i utjecaj produkta (propanola i NADP+) na početnu brzinu reakcije. Kinetika reakcije je opisana dvosupstratnom Michaelis-Menteničinom kinetikom s kompetitivnom inhibicijom produktima, a parametri su procijenjeni nelinearnom regresijom. Ispitivanje aktivnosti enzima u povratnoj reakciji pokazalo je da KRED ne katalizira reakciju oksidacije propanola. Provedena su mjerenja utjecaja propanala na stabilnost biokatalizatora, variranjem koncentracije propanala u reaktoru. Na temelju izmjerenih ovisnosti postavljen je matematički model enzimske reakcije, te su napravljene simulacije procesa.
Abstract (english)
In this paper, the kinetics of ketoreductase (KRED) was investigated in the reaction of propionaldehyde reduction. For its activity, this enzyme requires the use of coenzyme nicotinamide-adenine dinucleotide phosphate (NADPH). The kinetics was determined by the initial reaction rate method, by measuring dependence on reaction rate on substrate concentration (propionaldehyde and NADPH). Also, the impact of product (propanol and NADP+) on initial reaction rate was examined. Reaction kinetics was described by double substrate Michaelis–Menten kinetics with include competitive product inhibition. Kinetic parameters of Michaelis-Menten equation were estimated by nonlinear regression The enzyme did not show any measurable activity in reverse reaction, oxidation of propanol. Stability of enzyme was measured in the presence of different concentrations of propionaldehyde. Based on the experimental data mathematical model was set and the process simulations have been done.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:718130
Study programmeTitle: Environmental Engineering - Undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2019-07-12
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Created on2023-05-26 10:00:43