Due to their nature and compatibility with non-polar organic compounds, organic solvents are used in most synthetic reactions on an industrial scale. Such chemical processes often require multistep reactions and contaminating chemical reagents. A biotechnological alternative to such chemical processes includes enzymes that act as biocatalysts, enable the use of biologically based raw materials and reduce the number of reaction steps which can be accompanied by cheaper production. As a result, high quality products are obtained in an economically and technologically competitive and sustainable manner. Although biocatalytic processes are considered as an ideal solution for the development of sustainable production systems and despite tremendous advances in the discovery and development of enzymes in the field of molecular biology, the often achieved low productivity of the biocatalytic process together with the low stability and activity of enzymes, prevents their potential from being realized in industrial applications processes. Halohydrin dehalogenases (HHDH) are very rare enzymes that naturally participate in the bacterial degradation of halogenated compounds and due to their catalytic potential they belong to industrially relevant biocatalysts. This work uses a C-type enzyme from the halohydrin dehalogenase group (HheC) isolated from a genetically modified bacterium Escherichia coli. The activity of the HheC enzyme, in the presence of twelve different organic solvents, was tested using para-nitrostyrene oxide (PNSO) with bromide ions in the ring-opening reaction with the formation of para-nitro-2-bromo-1-phenylethanol (PNSHH). Enzyme activity was determined using the initial reaction rates method and the concentrations of PNSHH and PNSO were determined using a prepared calibration curves. The prepared samples were analyzed using an HPLC device and the influence of volume ratios of organic solvents on the specific activity of the enzyme was shown graphically. The obtained values of logP and c50 of organic solvents are presented in a table. Due to the toxicity or flammability of many organic solvents, there are green alternatives in terms of green organic solvents that are increasingly being used. Four green organic solvents obtained from natural sources were tested in this paper.