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undergraduate thesis
Priprema biokatalizatora
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2023. urn:nbn:hr:149:002930
Vrus, Lea
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Vrus, L. (2023). Priprema biokatalizatora (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:002930

Vrus, Lea. "Priprema biokatalizatora." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:149:002930

Vrus, Lea. "Priprema biokatalizatora." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:149:002930

Vrus, L. (2023). 'Priprema biokatalizatora', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:149:002930

Vrus L. Priprema biokatalizatora [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2023 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:149:002930

L. Vrus, "Priprema biokatalizatora", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:149:002930

Metadata
TitlePriprema biokatalizatora
Title (english)Preparation of biocatalysts
AuthorLea Vrus
MentorMartina Sudar (mentor)
Committee memberMartina Sudar (predsjednik povjerenstva)
Committee memberAnita Šalić (član povjerenstva)
Committee memberMonika Šabić Runjavec (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2023-09-14, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
Biokatalizatori, enzimi, se koriste za ubrzavanje biokemijskih reakcija u živim organizmima. Upotrebljavaju se u velikom rasponu industrija, poput farmaceutske, agrokemijske, prehrambene te u raznim znanstvenim granama. Zahvaljujući toj industrijskoj važnosti biokatalizatora razvijene su razne metode njihove pripreme. Mikrobna fermentacija, kultura biljnih i životinjskih stanica, uz DNK rekombinantnu tehnologiju omogućuju olakšanu sintezu specifičnih enzima. Krajnji produkt, biokatalizator, dobiva se tek nakon pročišćavanja koje uključuje izolaciju enzima iz žive stanice i daljnje postupke poput centrifugiranja i isoljavanja. Tako izolirani enzim podvrgava se metodama poput gel elektroforeze i kromatografije kako bi se uklonili svi neželjeni produkti.
Abstract (english)
Biocatalysts, enzymes, are used to accelerate biochemical reactions in living organisms. They are used in a wide range of industries, such as pharmaceutical, agrochemical, food and in various scientific branches. Thanks to this industrial importance of biocatalysts, various methods of their preparation have been developed. Microbial fermentation, the culture of plant and animal cells, along with DNA recombinant technology enable an easier synthesis of specific enzymes. The final product, the biocatalyst, is obtained after purification, which includes the isolation of the enzyme from the living cell and further procedures such as centrifugation and salting out. The isolated enzyme is then subjected to methods such as gel electrophoresis and chromatography in order to remove all unwanted products.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:002930
Study programmeTitle: Applied Chemistry - Undergraduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry
Course: Applied organic chemistry
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-09-15 11:58:29