prikaz prve stranice dokumenta Karakterizacija autohtonih mikroorganizama s visokom sposobnošću razgradnje ugljikovodika iz naftom onečišćenog tla
Embargo period 2026-10-01
master's thesis
Karakterizacija autohtonih mikroorganizama s visokom sposobnošću razgradnje ugljikovodika iz naftom onečišćenog tla
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2023. urn:nbn:hr:149:966807
Todorović, Nolla
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

Cite this document

Todorović, N. (2023). Karakterizacija autohtonih mikroorganizama s visokom sposobnošću razgradnje ugljikovodika iz naftom onečišćenog tla (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:966807

Todorović, Nolla. "Karakterizacija autohtonih mikroorganizama s visokom sposobnošću razgradnje ugljikovodika iz naftom onečišćenog tla." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:149:966807

Todorović, Nolla. "Karakterizacija autohtonih mikroorganizama s visokom sposobnošću razgradnje ugljikovodika iz naftom onečišćenog tla." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:149:966807

Todorović, N. (2023). 'Karakterizacija autohtonih mikroorganizama s visokom sposobnošću razgradnje ugljikovodika iz naftom onečišćenog tla', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:149:966807

Todorović N. Karakterizacija autohtonih mikroorganizama s visokom sposobnošću razgradnje ugljikovodika iz naftom onečišćenog tla [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2023 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:149:966807

N. Todorović, "Karakterizacija autohtonih mikroorganizama s visokom sposobnošću razgradnje ugljikovodika iz naftom onečišćenog tla", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:149:966807

Metadata
TitleKarakterizacija autohtonih mikroorganizama s visokom sposobnošću razgradnje ugljikovodika iz naftom onečišćenog tla
Title (english)Characterization of autochthonous microorganisms with high ability to degrade hydrocarbons from oil contaminated soils
AuthorNolla Todorović
MentorMarija Vuković Domanovac (mentor)
Committee memberMarija Vuković Domanovac (predsjednik povjerenstva)
Committee memberZvjezdana Findrik Blažević (član povjerenstva)
Committee memberMonika Šabić Runjavec (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2023-09-13, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
Kako potreba za naftom i njenim proizvodima raste, tako dolazi i do sve češćih onečišćenja okoliša prilikom raznih aktivnosti tijekom njezine uporabe. Zbog svog negativnog utjecaja na sav živi svijet, naftne ugljikovodike nužno je ukloniti iz okoliša. Jedan od postupaka za remedijaciju tla onečišćenog naftom je bioremedijacija. Bioremedijacija je okolišno i ekonomski prihvatljiv postupak za uklanjanje prisutnog onečišćenja u kojem mikroorganizmi i njihovi enzimi imaju ključnu ulogu. Kako bi se povećala učinkovitost bioremedijacije potrebno je selektirati mikroorganizme koji pokazuju afinitet za razgradnju naftnih ugljikovodika. U ovom radu provedena je izolacija, identifikacija i karakterizacija autohtonih mikrobnih kultura iz uzorka tla onečišćenog sirovom naftom u šaržnim uvjetima u periodu 28 dana. Dobivene vrijednosti CFU su se kretale u rangu od 1,20 × 10^7 do 1,15 × 10^9/g tla. Rezultati pokazuju da je aktivnost bakterija i gljiva bila najveća pri pH-vrijednosti 7,25 i 6,5. Na temelju morfologije, biokemijskih testova i MALDI TOF analize identificirane su bakterijske vrste: Bacillus megaterium, Bacillus licheniformis, Bacillus cereus i Pseudomonas chlororaphis.
Abstract (english)
As the demand for petroleum and petroleum products increases, so does the pollution caused by the various activities associated with the use of petroleum. Because of their negative impact on all living things, petroleum hydrocarbons must be removed from the environment. One of the methods of remediation of oil polluted soil is bioremediation. Bioremediation is an ecologically and economically viable method of removing contaminated sites, in which microorganisms and their enzymes play a key role. In order to increase the efficiency of bioremediation, it is necessary to select microorganisms that have affinity for degradation of petroleum hydrocarbons. In this work, the isolation, identification and characterization of autochthonous microbial cultures from a petroleum contaminated soil sample was carried out under batch conditions over a period of 28 days. Determinated CFU values ranged from 1.20 × 10^7 to 1.15 × 10^9/g soil. The results show that the activity of bacteria and fungi was highest at pH 7.25 and 6.5. Based on morphology, biochemical tests and analysis of MALDI TOF, bacterial species were identified: Bacillus megaterium, Bacillus licheniformis, Bacillus cereus, and Pseudomonas chlororaphis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:966807
Study programmeTitle: Environmental Engineering - Graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2026-10-01
Terms of useLicence In copyright icon
Created on2023-09-21 12:05:44