prikaz prve stranice dokumenta Optimization of reaction conditions of enzyme-catalyzed rhododendrol glycoside hydrolysis
Embargo period 2025-10-01
master's thesis
Optimization of reaction conditions of enzyme-catalyzed rhododendrol glycoside hydrolysis
Zagreb, Split: University of Zagreb, Faculty of Chemical Engineering and Technology, 2023. urn:nbn:hr:149:267066
Habazin, Antonia
University of Zagreb
Faculty of Chemical Engineering and Technology
University of Split
Faculty of Chemistry and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

Cite this document

Habazin, A. (2023). Optimization of reaction conditions of enzyme-catalyzed rhododendrol glycoside hydrolysis (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:267066

Habazin, Antonia. "Optimization of reaction conditions of enzyme-catalyzed rhododendrol glycoside hydrolysis." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology; University of Split, Faculty of Chemistry and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:149:267066

Habazin, Antonia. "Optimization of reaction conditions of enzyme-catalyzed rhododendrol glycoside hydrolysis." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology; University of Split, Faculty of Chemistry and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:149:267066

Habazin, A. (2023). 'Optimization of reaction conditions of enzyme-catalyzed rhododendrol glycoside hydrolysis', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology; University of Split, Faculty of Chemistry and Technology, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:149:267066

Habazin A. Optimization of reaction conditions of enzyme-catalyzed rhododendrol glycoside hydrolysis [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; Split: University of Split, Faculty of Chemistry and Technology; 2023 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:149:267066

A. Habazin, "Optimization of reaction conditions of enzyme-catalyzed rhododendrol glycoside hydrolysis", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb; University of Split, Faculty of Chemistry and Technology, Split, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:149:267066

Metadata
TitleOptimization of reaction conditions of enzyme-catalyzed rhododendrol glycoside hydrolysis
Title (croatian)Optimiranje reakcijskih uvjeta hidrolize rododendrol glikozida katalizirane enzimima
AuthorAntonia Habazin
MentorAna Vrsalović Presečki (mentor)
Committee memberAna Vrsalović Presečki (predsjednik povjerenstva)
Committee memberHrvoje Kušić (član povjerenstva)
Committee memberMarin Kovačić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
GranterUniversity of Split
Faculty of Chemistry and Technology
Split
Defense date and country2023-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
Rhododendrol glycosides are found in birch bark and can serve as a starting substrate for the production of raspberry ketone, via a hydrolysis reaction by the enzyme glucosidase, producing the intermediates (R)- and (S)-rhododendrol, followed by oxidation to the desired product. Natural properties of raspberry ketone include its use in cosmetics and perfumes, its anti-inflammatory and antioxidant abilities, its ability to prevent cancer, and its ability to combat insulin resistance. In this study, β-glucosidase from almonds was used to facilitate the hydrolysis of rhododendrol glycoside (betuloside). Different types of reactors were used to carry out the reaction, including a batch, a repetitive batch and a continuous enzyme membrane reactor (EMR). The kinetics of the hydrolysis was described by single-substrate Michaelis-Menten kinetics. The reaction products, rhododendrol and glucose, were found to inhibit the reaction in a competitive manner. During the implementation of the process, deactivation of the enzyme occurred and was described by the second-order kinetics. A batch reactor and an EMR were used to set up and validate a mathematical model of the process. In batch reactors a conversion of 100% was achieved. The highest productivity and stability of the enzyme was obtained when the reaction was carried out in a continuous EMR.
Abstract (croatian)
Glikozidi rododendrola nalaze se u kori breze i mogu djelovati kao početni supstrat za proizvodnju ketona maline, reakcijom hidrolize pomoću enzima glukozidaze koja proizvodi međuprodukte (R)- i (S)-rododendrola, nakon čega slijedi oksidacija do željenog produkta. Prirodna svojstva ketona maline uključuju njegovu upotrebu u kozmetici i parfemima, njihove protuupalne i antioksidativne sposobnosti, njihovu sposobnost sprječavanja raka, borbe protiv inzulinske rezistencije. U ovom diplomskom radu, β-glukozidaza iz badema korištena je za kataliziranje reakcije hidrolize rododendrol glikozida (betulozida) pri čemu nastaju dva enantiomera rododendrola. Za provođenje reakcije korišteni su različiti tipovi reaktora, uključujući kotlasti reaktor, ponovljivi kotlasti reaktor i kontinuirani enzimski membranski reaktor (EMR). Kinetika reakcije je opisana jednosupstratnom Michaelis-Menten kinetikom. Utvrđeno je da produkti reakcije, rododendrol i glukoza, inhibiraju reakciju na kompetitivni način. Prilikom provedbe procesa uočena je deaktivacija enzima koja je opisana kinetikom drugog reda. Kotlasti reaktor i EMR korišteni su za postavljanje i validaciju matematičkog modela procesa. U kotlastim reaktorima dobivena je konverzija supstrata od 100%. Najveća produktivnost i stabilnost enzima postignuta je prilikom provođenja reakcije u kontinuiranom EMR.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:149:267066
ProjectNumber: 101000560
Title: Rapid discovery and development of enzymes for novel and greener consumer products
Acronym: RADICALZ
Leader: Zvjezdana Findrik Blažević
Jurisdiction: eu
Funder: EC
Funding stream: H2020
Study programmeTitle: Chemical and environmental technology - graduate - university
Study programme type: university
Study level: graduate
Academic / professional title: sveučilišni/a magistar/magistra inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-10-01
Terms of useLicence In copyright icon
Created on2023-11-22 11:01:39