The supercritical fluid chromatography (SFC) is experiencing a revival due to the need to analyze samples of groving complexitiy. Such analytical systems allow for the use of a wide range of stationary phases and detectors, while commonly used mobile phase is CO2. The variety of choice of detectors and stationary phases makes SFC a good basis for the development of multi-dimensional chromatographic systems. SFC × GC and SFC × HPLC two-dimensional systems are well known, while SFC × SFC systems are still under development. One of the main obstacles to the faster development of two-dimensional chromatography is the analyte transfer between dimensions. A new module for the inter-dimensional transfer of the analyte was constructed, to allow a better insight to the problem of inter-dimensional transfer of the analyte. The principal characteristics of the module is the ability to control the flow values and the pressure values of the mobile chromatographic phase. The module was used to determine the influence of these values on the quality of two-dimensional fluid chromatography under supercritical conditions. The hypothesis of the need for independent control of the mobile phase during the transfer has proven correct and new approach, in developing the actual sample analysis method, has been tried. The developed method uses a high flow rate with a low mobile phase pressure during interdimensional transfer of analyte leading to compression analites in the second dimension, thus increasing the resolution in the second dimension and in the entire analytical system. The developed method was applied in the analysis of actual samples, confirming the improvements in the quality of the analytical data and the applicability of the method in the analysis of complex samples.