The aim of this work was to apply deep eutectic solvents, "green solvents", for the extraction of bioactive components from immortelle as a substitute for conventional organic solvents, which may be harmful to both the environment and humans. DESs based on choline chloride (ChCl) and ethylene glycol (EG) and betaine (B) and ethylene glycol (EG) were tested. HBA:HBD molar ratios of 1:3, 1:4 and 1:5 were used for both types of DESs. Polyphenolic compounds were extracted: phenolic acids, flavonoids and tannins from immortelle. Immortelle (lat. Helichrysum italicum) is a valuable medicinal plant that is best known among the wider population for its ability to regenerate the skin and is present in many cosmetic products of high-end brands as well as in the perfume industry due to its characteristic smell. The determination of the content of polyphenolic compounds was carried out according to the procedures of the European Pharmacopoeia, by UV/VIS spectrophotometry. For phenolic acids and flavonoids, the extraction capacity was determined by a multi-stage extraction process. Furthermore, the concentration of chicory acid, a derivative of caffeic acid, in different immortelle flower extracts was determined using the high performance liquid chromatography (HPLC) method. Based on the experimental data on the extraction of phenolic acids, DES B:EG 1:5 with the highest added mass of immortelle dry matter proved to be the best. B:EG 1:3 proved to be the worst, with the smallest added mass of immortelle dry matter. For the determination of flavonoids, B:EG 1:3 with the largest mass was the best, and ChCl:EG 1:5 with the largest mass of immortelle was the worst. ChCl:EG 1:4 with the smallest mass of immortelle was the best, and ChCl:EG 1:3 with the largest mass was the worst. Due to excessive fluctuations in the content of tannins, the extraction capacity was determined only for phenolic acids and flavonoids. It was concluded that the equilibrium extraction capacity was reached in the 1st stage for both phenolic acids and flavonoids. In addition, the concentration of chicory acid was determined by high-performance liquid chromatography. The sample ChCl:EG 1:5 with the highest mass had the highest concentration, and ChCl:EG 1:4 with the lowest mass of immortelle dry matter had the lowest concentration.