Composite porous materials combine different components in order to achieve enhanced properties that exceed the capabilities of individual materials. Composites containing bioactive glass and biodegradable polymers are of particular interest due to their ability to simultaneously provide structural support, stimulate tissue regeneration and gradually degrade within the body. Because of its biocompatibility and ability to interact with biological tissue, bioactive glass is widely used in regenerative medicine, especially in the form of a three-dimensional scaffold for tissue engineering. The objective of this thesis is to prepare composite three-dimensional scaffolds using a highly porous matrix composed of bioglass and chitosan-based spheres. Such scaffolds are prepared using the foam replica method. The primary task was to find suitable procedures and optimal parameters to successfully stick the spheres onto the three-dimensional glass matrix. Previous research has confirmed the optimal parameters for the synthesis of microspheres using the electrospraying method. To impregnate the spheres on to the scaffold, suspensions with 5% or 10% of the sphere mass per volume of solvent are prepared. A sodium alginate solution of 0.5, 1 and 2 w/v % was prepared for glueing the spheres to the scaffold. The effectiveness of the process was evaluated by the loss of mass after annealing and through SEM analysis. The stability of the bioactive glass scaffold was confirmed. Its structure is complex and highly porous, with a rough surface that facilitates integration with biological tissue. The results showed that sample BG-1ALG contains the thickest polymer coating, which indicates the optimal concentration of alginate to achieve the best adhesion of microspheres. It was concluded that the sample BG-1ALG-10SF is the most effective, combining an adequate amount of spheres and a highly porous structure, which makes it suitable for medical applications.