The aim of the study was to synthesize and characterize compound (S)-11-(2-heptanamido-3-(1H-imidazol-4-yl)propanamido)-N-(pyridin-4-ylmethyl)undecanamide hydrochloride (7a), a derivative of L-histidine (L-His), as a potential low molecular weight gelator (LMWG) of amphiphilic properties. The target compound was examined for its molecular self-assembly potential, i.e. formation of hydrogels, as well as the ability to coordinate Zn2+. Furthermore, the catalytic activity of the newly synthesized compound was investigated through the reaction of hydrolytic conversion of p-nitrophenyl acetate (p-NPA) to p-nitrophenol (p-NP), which models the process of natural ester hydrolysis reaction in biological systems. Previously synthesized compounds, structural analogues of compound 7a with altered amino acids (7b – L-phenylalanine derivative, 7c – L-phenylglycine derivative and 7d – L-tryptophan derivative) were also investigated for the possibility of forming complexes with Zn2+ and catalytic action. Measurements were carried by UV/Vis spectroscopy. Newly synthesized compound 7a contains a long aliphatic chain, amino acid L-His and terminal 4-picolylamine. Its preparation was successfully carried out in 7 steps: 1. protection of 11-aminoundecanoic acid (AUDA) with a Boc group (1); 2. activation of compound 1 by N-hydroxysuccinimide (NHS) (3); 3. substitution of the succinimide group of compound 3 for 4-picolylamine (4); 4. removal of the Boc-protective group from compound 4 (5); 5. synthesis of compound 2 from L-His and heptanoyl-chloride; 6. condensation reaction between compounds 5 and 2 (6); 7. conversion of compound 6 into a hydrochloride salt (7a). The targeted compound 7a was evaluated for the possibility of gel formation with deionized water and phosphate buffer, whereby a colloidal solution was formed, while compounds 7b, 7c and 7d successfully form hydrogels. All tested compounds showed the possibility of Zn2+ coordination, as well as catalytic activity in the hydrolytic reaction of p-NP formation from p-NPA, with necessary additional method optimization.