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master's thesis
Enzimatska sinteza 6-cijano-4-ketoheksanske kiseline
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2016. urn:nbn:hr:149:584402
Lešnjak, David
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

Cite this document

Lešnjak, D. (2016). Enzimatska sinteza 6-cijano-4-ketoheksanske kiseline (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:584402

Lešnjak, David. "Enzimatska sinteza 6-cijano-4-ketoheksanske kiseline." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2016. https://urn.nsk.hr/urn:nbn:hr:149:584402

Lešnjak, David. "Enzimatska sinteza 6-cijano-4-ketoheksanske kiseline." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2016. https://urn.nsk.hr/urn:nbn:hr:149:584402

Lešnjak, D. (2016). 'Enzimatska sinteza 6-cijano-4-ketoheksanske kiseline', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:149:584402

Lešnjak D. Enzimatska sinteza 6-cijano-4-ketoheksanske kiseline [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2016 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:149:584402

D. Lešnjak, "Enzimatska sinteza 6-cijano-4-ketoheksanske kiseline", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:149:584402

Metadata
TitleEnzimatska sinteza 6-cijano-4-ketoheksanske kiseline
Title (english)Enzymatic synthesis of 6-cyano-4-ketohexanoic acid
AuthorDavid Lešnjak
MentorĐurđa Vasić-Rački (mentor)
Committee memberĐurđa Vasić-Rački (predsjednik povjerenstva)
Committee memberZvjezdana Findrik Blažević (član povjerenstva)
Committee memberMarija Vuković Domanovac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2016-07-12, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Reaction Engineering
Abstract
Cilj ovog rada bio je istražiti enzimatsku reakciju kataliziranu sintazom 2-sukcinil-5- enolpiruvil-6-hidroksi-3-cikloheksen-1-karboksilne kiseline uz kratkolančani α-ketoglutarat kao donorski supstrat, akrilonitril kao akceptorski supstrat, tiamin difosfat kao koenzim, te magnezijev sulfat kao kofaktor. Istraživan je utjecaj različitih vrijednosti temperature, pH i koncentracije svih reakcijskih komponenti na brzinu reakcije. Iz prikupljenih eksperimentalnih podataka su procijenjeni kinetički parametri Michaelis-Menteničinog modela koji je kasnije validiran eksperimentom u kotlastom reaktoru.
Abstract (english)
The aim of this work was to investigate the enzymatic reaction catalyzed by 2-succinyl-5- enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase with short-chain α- ketoglutarate as the donor substrate, acrylonitrile as the acceptor substrate, thiamine diphosphate as the coenzyme, and magnesium sulfate as the cofactor. The effect of different temperatures, pH values and concentrations of all reaction compounds on reaction rate has been investigated. From the gathered experimental data kinetic parameters of the Michaelis-Menten model type have been estimated. The mathematical model that was developed has been experimentally validated.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:149:584402
ProjectNumber: 635595
Title: Sustainable industrial processes based on a C-C bond-forming enzyme platform
Acronym: CARBAZYMES
Leader: Zvjezdana Findrik Blažević
Jurisdiction: eu
Funder: EC
Funding stream: H2020
Study programmeTitle: Applied Chemistry - Graduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry
Course: Advanced materials and technologies
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra primijenjene kemije (magistar/magistra primijenjene kemije)
Type of resourceText
File originBorn digital
Access conditionsClosed access
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Created on2017-04-24 09:55:37