The goal of this work was to extract free glycerol from produced biodiesel in a reciprocating plate extractor using deep eutectic solvent choline-chloride/ethylen-glycol (molar ratio 1:2,5). The properties of prepared deep eutectic solvent, such as refractive indeks, pH value, electrical conductivity and viscosity were measured as the function of temperature and water content. Biodiesel was produced from „Zvijezda“ sunflower oil, both fresh and the one used to fry pommes frites. A small amount of biodiesel from oil used to make donuts and paprika flavoured pommes frites was also produced, in order to compare the products made from different kinds of waste frying oil. The effect of hydrodinamic conditions, solvent to biodiesel ratio and duration of extraction on its efficiency were tested during batch experiments. Continuous experiments were conducted under constant solvent and biodiesel flowrates with different number of pulsations per minute. All raw materials and biodiesel samples were qualitatively analysed using FTIR spectroscopy. Glycerol and glycerides content of selected biodiesel samples were quantitatively determined using gas chromatography. The results have demonstrated that hydrodinamic conditions, solvent to biodiesel ratio and extraction duration have a largerly negligible impact on the efficiency of free glycerol removal in the tested range of conditions and that the efficiency is relatively high for all conducted experiments. The efficiency of free glycerol removal under the same batch extraction conditions was the lowest (cca 87 %) for biodiesel produced from fresh oil, while the efficiency of all three waste oil biodiesels was almost equal (around 97 %). The efficiency was only slightly lower during continuous extraction. Free glycerol content was below the limit, but the total glycerol and glycerides content was too high to comply with the standard. ChCl-EG (1:2,5) deep eutectic solvent has proved to be succesfull for free glycerol extraction, but further modification of methods for raw material and biodiesel processing will be needed to fully comply with the standard.