prikaz prve stranice dokumenta Ispitivanje aktivnosti analoga sintaza N-acetilneuraminske kiseline na neprirodne supstrate
Embargo period 2026-10-01
undergraduate thesis
Ispitivanje aktivnosti analoga sintaza N-acetilneuraminske kiseline na neprirodne supstrate
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2024. urn:nbn:hr:149:802459
Drandić, Lucija
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

Cite this document

Drandić, L. (2024). Ispitivanje aktivnosti analoga sintaza N-acetilneuraminske kiseline na neprirodne supstrate (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:802459

Drandić, Lucija. "Ispitivanje aktivnosti analoga sintaza N-acetilneuraminske kiseline na neprirodne supstrate." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2024. https://urn.nsk.hr/urn:nbn:hr:149:802459

Drandić, Lucija. "Ispitivanje aktivnosti analoga sintaza N-acetilneuraminske kiseline na neprirodne supstrate." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2024. https://urn.nsk.hr/urn:nbn:hr:149:802459

Drandić, L. (2024). 'Ispitivanje aktivnosti analoga sintaza N-acetilneuraminske kiseline na neprirodne supstrate', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 13 January 2025, https://urn.nsk.hr/urn:nbn:hr:149:802459

Drandić L. Ispitivanje aktivnosti analoga sintaza N-acetilneuraminske kiseline na neprirodne supstrate [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2024 [cited 2025 January 13] Available at: https://urn.nsk.hr/urn:nbn:hr:149:802459

L. Drandić, "Ispitivanje aktivnosti analoga sintaza N-acetilneuraminske kiseline na neprirodne supstrate", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:149:802459

Metadata
TitleIspitivanje aktivnosti analoga sintaza N-acetilneuraminske kiseline na neprirodne supstrate
Title (english)Screening of N-acetylneuraminic acid synthase analogues on unnatural substrates
AuthorLucija Drandić
MentorZvjezdana Findrik Blažević (mentor)
Committee memberZvjezdana Findrik Blažević (predsjednik povjerenstva)
Committee memberMartina Sudar (član povjerenstva)
Committee memberMarija Vuković Domanovac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2024-09-17, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
Sijalinske kiseline mogu posredovati ili modulirati u širokom spektru fizioloških i patoloških procesa zbog svoje sveprisutne distribucije i strateške lokacije. U posljednjem desetljeću postignut je značajan napredak u proučavanju glikana na ljudskim stanicama, kao i njihove uloge u interakcijama među stanicama. Ugljikohidrati igraju mnogobrojne uloge u različitim organizmima, uključujući razvoj, signalizaciju, interakcije između domaćina i parazita te imunološku funkciju. Iako je provedeno mnogo istraživanja, biologija sijalinske kiseline je još uvijek rastuće područje velikog potencijala čije su primjene tek započele. U ovom radu provedena je kondenzacijska reakcija šećera (L-arabinoza, D-galaktoza, D-liksoza, D-(+)-manoza) i fosfoenolpiruvata uz prisutnost metalnog kofaktora Co2+. Reakcije su katalizirane analozima sintaze N-acetinneuraminske kiseline (PNH2, PNH5), umjetno dobiveni na temelju sličnosti sa sintazom neuraminske kiseline iz bakterije Neisseria meningitidis. Produkt reakcija su različite sijalinske kiseline. Provedena su ispitivanja s ekstraktom stanica te s pročišćenim enzimom radi potvrde aktivnosti enzima i nastajanja analognih produkata sijalinske kiseline. Uočena je najveća kompatibilnost enzima PNH5 s testiranim šećerima.
Abstract (english)
Sialic acids can mediate or modulate a wide range of physiological and pathological processes due to their omnipresent distribution and strategic location. In the past decade, significant progress has been made in the study of glycans on human cells, as well as their role in cell-cell interactions. Carbohydrates play numerous roles in various organisms, including development, signaling, host-parasite interactions, and immune function. Although much research has been conducted, sialic acid biology is still an emerging field with great potential, while its applications are just beginning. In this study, a condensation reaction of sugars (L-arabinose, D-galactose, D-lyxose, D-(+)-mannose) and phosphoenolpyruvate in the presence of the metal cofactor Co2+. The reactions were catalyzed by analogs of N-acetylneuraminic acid synthase (PNH2, PNH5), which were artificially derived based on their similarity to the neuraminic acid synthase from the bacterium Neisseria meningitidis. The product of the reactions are different sialic acids. Assays were conducted on both cell-free extracts and purified enzyme to confirm the enzyme activity and the formation of sialic acid analogs. The highest compatibility of the enzyme PNH5 with the tested sugars was observed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:802459
ProjectNumber: 956631 — CC-TOP — H2020-MSCA-ITN-2020
Title: C-C Bond Formation Using Top Performing Enzymes
Acronym: C-C Top
Leader: Zvjezdana Findrik Blažević
Jurisdiction: eu
Funder: Europska unija
Funding stream: Horizon 2020
Study programmeTitle: Applied Chemistry - Undergraduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry
Course: Environmental chemistry and green technologies
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2026-10-01
Terms of useLicence In copyright icon
Created on2024-11-19 09:10:27