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undergraduate thesis
Politiramin kao osnova senzorske površine
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2015. urn:nbn:hr:149:248483
Marcinek, Saša
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Marcinek, S. (2015). Politiramin kao osnova senzorske površine (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:248483

Marcinek, Saša. "Politiramin kao osnova senzorske površine." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2015. https://urn.nsk.hr/urn:nbn:hr:149:248483

Marcinek, Saša. "Politiramin kao osnova senzorske površine." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2015. https://urn.nsk.hr/urn:nbn:hr:149:248483

Marcinek, S. (2015). 'Politiramin kao osnova senzorske površine', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:149:248483

Marcinek S. Politiramin kao osnova senzorske površine [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2015 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:149:248483

S. Marcinek, "Politiramin kao osnova senzorske površine", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:149:248483

Metadata
TitlePolitiramin kao osnova senzorske površine
Title (english)Polytyramine as a base for sensor preparation
AuthorSaša Marcinek
MentorStjepan Milardović (mentor)
Committee memberStjepan Milardović (predsjednik povjerenstva)
Committee memberMarijana Kraljić Roković (član povjerenstva)
Committee memberIrena Kereković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2015-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Inorganic Chemistry
Abstract
U ovom radu konstruiran je senzor za određivanje albumina iz humanog seruma (HSA) temeljen na politiraminom modificiranoj zlatnoj elektrodi. Imobilizacija politiramina izvedena je elektropolimerizacijom 1 M otopine NaOH uz dodatak 0,1 M tiramina. Funkcionalizacija površine provedena je s nitrilotriocenom kiselinom (NTA). Amino skupine politiramina aktivirane su uranjanjem u 25%-tnu otopinu glutaraldehida, što omogućava stvaranje veza između politiramina i NTA. Nakon vezanja NTA, elektroda je dodatno modificirana s Cu2+ i Ni2+ ionima. Karakterizacija zlatne elektrode provedena je cikličkom voltametrijom (CV) i elektrokemijskom impedancijskom spektroskopijom (EIS) u 0,08 M otopini KCl koja je sadržavala 1 mM K4[Fe(CN)6]/K3[Fe(CN)6]. Postojanje slojeva na elektrodi dodatno je potvrđeno mjerenjem na kvarcnoj kristalnoj mikrovagi (QCM). Analitička primjena modificirane elektrode testirana je s QCM-om. Rezultati su pokazali kako je NTA-Cu kompleks dobra osnova za određivanje HSA, dok se NTA-Ni kompleks nije pokazao kao optimalna senzorska konfiguracija za odabrani analit.
Abstract (english)
The aim of this work was development of a sensor for human serum albumin determination (HSA) based on gold electrode modified with polytyramine. Polytyramine immobilization was performed by electropolymerization in 1 M solution of NaOH containing 0.1 M of tyramine. Surface functionalization was carried out with nitrilotriacetic acid (NTA). Amino groups were activated by immersion in 25% glutaraldehyde solution. Subsequent to the NTA binding, electrode was additionally modified with Cu2+ and Ni2+ ions. Characterization of gold electrode was performed with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in the 0.08 M solution of KCl with 1 mM of K4[Fe(CN)6]/K3[Fe(CN)6]. Existence of the layers on the electrode was additionally confirmed with quartz Crystal Microbalance (QCM) measurements. Analytical performance of modified electrode was carried out with QCM. Results had shown that NTA-Cu complex can be used for detection of HSA while NTA-Ni complex was not optimal sensor configuration for the selected analyte.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:248483
Study programmeTitle: Environmental Engineering - Undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-09-15 10:29:16