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undergraduate thesis
Metode bioloških ispitivanja materijala pogodnih za umjetna tkiva
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2015. urn:nbn:hr:149:527649
Perčić, Maja
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Perčić, M. (2015). Metode bioloških ispitivanja materijala pogodnih za umjetna tkiva (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:527649

Perčić, Maja. "Metode bioloških ispitivanja materijala pogodnih za umjetna tkiva." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2015. https://urn.nsk.hr/urn:nbn:hr:149:527649

Perčić, Maja. "Metode bioloških ispitivanja materijala pogodnih za umjetna tkiva." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2015. https://urn.nsk.hr/urn:nbn:hr:149:527649

Perčić, M. (2015). 'Metode bioloških ispitivanja materijala pogodnih za umjetna tkiva', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:149:527649

Perčić M. Metode bioloških ispitivanja materijala pogodnih za umjetna tkiva [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2015 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:149:527649

M. Perčić, "Metode bioloških ispitivanja materijala pogodnih za umjetna tkiva", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:149:527649

Metadata
TitleMetode bioloških ispitivanja materijala pogodnih za umjetna tkiva
Title (english)Biological evaluation methods of materials suitable for artificial tissues
AuthorMaja Perčić
MentorMarica Ivanković (mentor)
Committee memberMarica Ivanković (predsjednik povjerenstva)
Committee memberJelena Macan (član povjerenstva)
Committee memberHrvoje Ivanković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2015-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Basic Technical Sciences
Materials
Abstract
Strategija inženjerstva tkiva oslanja se na kompleksne interakcije stanica i biomaterijala (nosača) kako bi se ostvarila uspješna regeneracija oštećenog tkiva. U tu svrhu biomaterijali su podvrgnuti ispitivanjima, naročito onima koja se odnose na procjenu biokompatibilnosti, odnosno sposobnosti materijala za obavljanje svoje terapeutske funkcije bez izazivanja nepoželjnih (upalnih) reakcija u tijelu pacijenta. In vitro testiranje je pokušaj simuliranja in vivo okruženja u strogo kontroliranim uvjetima. U ovom radu, dan je literaturni pregled izdvojenih bioloških metoda koje se upotrebljavaju za ispitivanje pogodnosti primjene materijala u inženjerstvu koštanog tkiva. Kolorimetrijske metode najčešće su korištene metode za brze testove citotoksičnosti materijala. Testovi kojima se utvrđuje umnažanje (proliferacija) i razvoj (diferencijacija) stanica temelje se na kvantitativnom određivanju specifičnih proteina (osteogenih markera), imunološkim fluorescentnim metodama bojanja te na histološkom snimanju biomaterijala.
Abstract (english)
Bone tissue engineering strategy relies on the complex interactions between cells and biomaterials (scaffolds) to achieve a successful regeneration of the bone tissue. For this purpose, biomaterials undergo testing, especially those for the assessment of material‘s biocompatibility, i.e. the ability of biomaterial to perform its desired therapeutically function, without eliciting any undesirable response. In vitro testing is an attempt to simulate the in vivo situation in a strictly controlled environment. In this work, several biological methods that are used for materials assessment as candidate in tissue engineering have been described. Colorimetric methods are mostly used for quick cytotoxicity assays of materials. Tests that determine the cell proliferation and differentiation are based on quantification of specific proteins (osteogenic markers), immunological fluorescence staining methods, and on histological evaluation of biomaterials.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:527649
Study programmeTitle: Chemical Engineering - Undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2016-09-19 12:40:25