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undergraduate thesis
Aktivnost halohidrin-dehalogenaze u reakciji nastajanja epoksida
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2016. urn:nbn:hr:149:773838
Knapić, Dominik
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

Cite this document

Knapić, D. (2016). Aktivnost halohidrin-dehalogenaze u reakciji nastajanja epoksida (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:773838

Knapić, Dominik. "Aktivnost halohidrin-dehalogenaze u reakciji nastajanja epoksida." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2016. https://urn.nsk.hr/urn:nbn:hr:149:773838

Knapić, Dominik. "Aktivnost halohidrin-dehalogenaze u reakciji nastajanja epoksida." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2016. https://urn.nsk.hr/urn:nbn:hr:149:773838

Knapić, D. (2016). 'Aktivnost halohidrin-dehalogenaze u reakciji nastajanja epoksida', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:149:773838

Knapić D. Aktivnost halohidrin-dehalogenaze u reakciji nastajanja epoksida [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2016 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:149:773838

D. Knapić, "Aktivnost halohidrin-dehalogenaze u reakciji nastajanja epoksida", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:149:773838

Metadata
TitleAktivnost halohidrin-dehalogenaze u reakciji nastajanja epoksida
Title (english)Activity of halohydrine dehalogenase in reaction of epoxide production
AuthorDominik Knapić
MentorĐurđa Vasić-Rački (mentor)
Committee memberĐurđa Vasić-Rački (predsjednik povjerenstva)
Committee memberAna Vrsalović Presečki (član povjerenstva)
Committee memberZvonimir Katančić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2016-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Reaction Engineering
Abstract
Istraživana je reakcija dehalogenacije 1,3 diklor-2-propanola koju katalizira enzim halohidrin-dehalogenaza (HHDH). Određena je aktivnost te optimizirana metoda određivanja aktivnosti enzima. Određivane su koncentracije α-kloroalkohola i epoksida plinskom kromatografijom s plameno ionizacijskim detektorom. Određena je i kinetika enzima pri pH 8. Reakcija dehalogenacije je provedena u kotlastom reaktoru. Rezultati su pokazali da enzim halohidrindehalogenaza nije specifičan na α-kloroalkohol. Kao optimalni pufer za mjerenje aktivnosti odabran je 0.1 M fosfatni pufer pH 8, a kao optimalna 10 mM koncentracija supstrata. Eksperimenti u reaktoru su pokazali da koncentracija epoksida koji je reakcijski produkt opada tijekom povedbe reakcije. Aktivnost enzima također opada, a nakon 4 sata ostaje stalna.
Abstract (english)
This reaserch deals with the reaction of dehalogenation of 1,3 dichloro-2-propanol that is catalyzed by the enzyme halohydrin-dehalogenase (HHDH). The activity of enzyme was determined. The method of determining the enzyme activity was optimized. The concentrations of α-chloroalcohol and epoxyde were determend with the gas chromatography which was supplied with the flame ionization detector The kinetics of the enzyme reaction at pH 8 was also determined. The reacton of dehalogenation was tested in a batch reactor. The results showed that the enzyme halohydrin-dehalogenase is not specific to α-chloroalcohol. As the optimum buffer for activity measurements 0.1 M phosphate buffer pH 8 was selected. 10 mM substrate concentration was chosen as optimal. Experiments in the reactor showed that the concentration of the epoxyde which is the reaction product decreases during the reaction. Enzyme activity also decreases, and after 4 hours remains constant.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:773838
Study programmeTitle: Environmental Engineering - Undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
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Created on2018-02-19 12:34:18