Due to increased consumption and production, pharmaceuticals are more ubiquitous in the environment. They can reach the soil, ground water and surface water and greatly affect on organisms in the ecosystem. No matter what they are present in low concentrations, their detection in the enviroment causes great concern for human health. In this paper, an analytical method for determination of thirteen pharmaceuticals (azithromycin, tiamulin, imatinib, febantel, torasemide, omeprazole, linezolid, praziquantel, etodolac, sulfamethazine, sulfafurazole, albendazole, levamisole) with different physical-chemical properties has been developed. Separation of pharmaceuticals from water was carried out by solid-phase extraction. The efficiency of the extraction was quantitatively determined by ultra high preformance liquid chromatography coupled with mass spectrometry as detector. The best extraction was achieved in following conditions: Oasis HLB sorbent at pH 5, methanol as elution solvent, 2 mL as the volume of the elution solvent and 100 mL as the volume of water sample. After optimizing the extraction conditions, the method has been validated. For investigated pharmaceuticals limit of quantification are ranged from 0,0025-0,5 μg/L and the limit of detection between 0,001-0,1 μg/L. After validation, previously developed method was applied to real samples, for determination of listed pharmaceutical in the pharmaceutical industry wastewaters. DryLab software was used for development of a quick and simple method of ultra high preformance liquid chromatography and to determine their optimal conditions (pH, temperature and composition of the mobile phase, etc).