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master's thesis
Procjena utjecaja eritromicina na bakteriju Pseudomonas putida izoliranu iz farmaceutske otpadne vode
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. urn:nbn:hr:149:070655
Miloloža, Martina
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Miloloža, M. (2018). Procjena utjecaja eritromicina na bakteriju Pseudomonas putida izoliranu iz farmaceutske otpadne vode (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:070655

Miloloža, Martina. "Procjena utjecaja eritromicina na bakteriju Pseudomonas putida izoliranu iz farmaceutske otpadne vode." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:149:070655

Miloloža, Martina. "Procjena utjecaja eritromicina na bakteriju Pseudomonas putida izoliranu iz farmaceutske otpadne vode." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:149:070655

Miloloža, M. (2018). 'Procjena utjecaja eritromicina na bakteriju Pseudomonas putida izoliranu iz farmaceutske otpadne vode', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 01 August 2024, https://urn.nsk.hr/urn:nbn:hr:149:070655

Miloloža M. Procjena utjecaja eritromicina na bakteriju Pseudomonas putida izoliranu iz farmaceutske otpadne vode [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2018 [cited 2024 August 01] Available at: https://urn.nsk.hr/urn:nbn:hr:149:070655

M. Miloloža, "Procjena utjecaja eritromicina na bakteriju Pseudomonas putida izoliranu iz farmaceutske otpadne vode", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:149:070655

Metadata
TitleProcjena utjecaja eritromicina na bakteriju Pseudomonas putida izoliranu iz farmaceutske otpadne vode
Title (english)Assessment of the impact of erythromycin on bacteria Pseudomonas putida isolated from pharmaceutical wastewater
AuthorMartina Miloloža
MentorMarija Vuković Domanovac (mentor)
Committee memberMarija Vuković Domanovac (predsjednik povjerenstva)
Committee memberZvjezdana Findrik Blažević (član povjerenstva)
Committee memberMartina Sudar (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2018-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Environmental Protection in Chemical Engineering
Abstract
Demografski rast stanovništva, širenje urbanih sredina, razvoj industrije te ubrzani način života, pokretačke su sile za povećanu upotrebu i proizvodnju farmaceutika. Različiti su izvori njihova dospijevanja u okoliš, a uslijed abiotičkih i biotičkih djelovanja mogu promijeniti svoju prvotnu strukturu. Potrebno je pratiti koncentracije farmaceutika u okolišu te standardnim metodama određivati njihov utjecaj na izložene organizme. Makrolidni se antibiotici navode kao štetni po okoliš uslijed velike potrošnje i duljeg zadržavanja u okolišu. U svrhu uklanjanja farmaceutika iz okoliša, proučavaju se razni postupci, no sve zastupljenije postaje iskorištavanje enzimatske sposobnosti mikroorganizama za njihovo uklanjanje. U ovome je radu provedena procjena utjecaja makrolidnog antibiotika eritromicina na bakterijsku kulturu Pseudomonas putidu izoliranu iz otpadne farmaceutske vode. Testovi toksičnosti provedeni pomoću bakterije Vibrio fischeri svrstavaju eritromicin kao slabo toksičnu tvar uz mogući rizik po okoliš. Istraživan je potencijal biorazgradnje eritromicina bakterijskom kulturom P. putidom s obzirom na rezultate testova osjetljivosti koji su pokazali otpornost kulture na raspon koncentracija eritromicina 0,2-2000 mg L^-1. Nakon uzgoja stanica baktrerijske kulture na Tryptic Soy Broth-u, praćena je aktivnost enzima esteraze u uvjetima bez i uz dodatak 0,2 mg L^-1 i 200 mg L^-1 eritromicina. Aktivnost enzima je rasla bez obzira na koncentraciju eritromicina, ali su pri 200 mg L^-1 uočene morfološke promjene stanica P. putide.
Abstract (english)
Demographic growth of population, urbanization, industrialization and hectic lifestyle are driving forces for increasing utilization of pharmaceuticals and their production. There are different sources of their entry into the environment and due to abiotic and biotic activity their primary structures can be changed. It is necessary to control concentration of pharmaceuticals in the environment and determine their impact on exposed organisms by standard methods. Macrolide antibiotics are harmful for the environment due to their large consumption and longer environmental persistence. In order to remove pharmaceuticals from the environment, different processes are being researched with emphasis on enzymatic capability of microorganisms. In this study, assessment of the macrolide antibiotic erythromycin on bacterial culture Pseudomonas putida isolated from pharmaceutical wastewater was investigated. Bioassays conducted with bacteria Vibrio fischeri classified erythromycin as low toxic substance with possible risk for the environment. Biodegradation potential of erythromycin with bacterial culture Pseudomonas putida was investigated with susceptibility tests which indicated the resistance of bacteria at range of erythromycin concentration from 0.2 to 2000 mg L^-1. After cultivation of bacterial culture on Tryptic Soy Broth, activity of enzyme esterase was determined in conditions without and with addition of 0.2 mg L^-1 and 200 mg L^-1 of erythromycin. Activity of enzyme increased regardless of erythromycin concentration, while morphological changes of P. putida were noticed at 200 mg L^-1 of erythromycin.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:070655
Study programmeTitle: Environmental Engineering - Graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-10-26 12:32:31