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master's thesis
Određivanje razgradnih produkata diazepama spektrometrijom masa visoke razlučivosti
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. urn:nbn:hr:149:817288
Karačić, Iva
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Karačić, I. (2018). Određivanje razgradnih produkata diazepama spektrometrijom masa visoke razlučivosti (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:817288

Karačić, Iva. "Određivanje razgradnih produkata diazepama spektrometrijom masa visoke razlučivosti." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:149:817288

Karačić, Iva. "Određivanje razgradnih produkata diazepama spektrometrijom masa visoke razlučivosti." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:149:817288

Karačić, I. (2018). 'Određivanje razgradnih produkata diazepama spektrometrijom masa visoke razlučivosti', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 17 November 2024, https://urn.nsk.hr/urn:nbn:hr:149:817288

Karačić I. Određivanje razgradnih produkata diazepama spektrometrijom masa visoke razlučivosti [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2018 [cited 2024 November 17] Available at: https://urn.nsk.hr/urn:nbn:hr:149:817288

I. Karačić, "Određivanje razgradnih produkata diazepama spektrometrijom masa visoke razlučivosti", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:149:817288

Metadata
TitleOdređivanje razgradnih produkata diazepama spektrometrijom masa visoke razlučivosti
Title (english)Determination of diazepam degradation products by high resolution mass spectrometry
AuthorIva Karačić
MentorDragana Mutavdžić Pavlović (mentor)
Committee memberDragana Mutavdžić Pavlović (predsjednik povjerenstva)
Committee memberTatjana Gazivoda Kraljević (član povjerenstva)
Committee memberMarija Vuković Domanovac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2018-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Analytic Chemistry
Abstract
Svaki lijek ili farmaceutik sadrži razna organska ili anorganska onečišćenja te razgradne produkte koji u lijeku obično zaostaju iz proizvodnog postupka, nastaju tijekom izrade ljekovitog oblika ili skladištenjem lijeka. Razgradni produkti mogu nastati djelovanjem svjetla, temperature, u prisutnosti vode ili promjenom pH, a mogu nastati i tijekom izrade ili čuvanja farmaceutskog dozirnog oblika. Njihova prisutnost, čak i u vrlo malim količinama, može utjecati na stabilnost, djelotvornost i sigurnost primjene lijeka. U ovom radu razvijena je brza i učinkovita metoda za analizu onečišćenja i razgradnih produkata farmaceutika diazepama u farmaceutskoj otopini. Za određivanje i kvantifikaciju diazepama i njegovih razgradnih produkata koristi se tekućinska kromatrografija ultravisoke djelotvornosti (UHPLC) spregnuta sa spektrometrijom masa (LC-MS/MS). Pomoću razvijene metode određeni su razgradni produkti nastali forsiranom razgradnjom diazepama u kiselini, lužini, vodikovom peroksidu i netretiranom uzorku, ispitan je utjecaj temperature i svjetlosti na razgradnju te su pretpostavljene strukture i način fragmentacije dobivenih razgradnih produkata. Za određivanje optimalnih uvjeta kromatografskog odjeljivanja metodom UHPLC (temperatura kolone, sastav pokretne faze, vremenski gradijent, pH) korišten je računalni program Drylab.
Abstract (english)
Each drug or pharmaceutical contains various organic or inorganic contaminants and degradable products that typically lag behind in drug during the manufacturing process, preparation of a medicated form or by storage of the drug. The degradable products can be generated under the influence of light, temperature, water or pH change, and may also occur during the preparation or storage of the pharmaceutical dosage form. Even in very small quantities, the presence of contamination can affect the efficacy and safety of drug use. In this study, a quick and efficient method for analysis of contaminants and degradation products of diazepam was developed. High efficiency liquid chromatography (UHPLC) coupled with mass spectrometry (LC-MS/MS) was used for determination and quantification of diazepam and his degradation products. Degradation products generated by forced degradation of diazepam in acid, alkaline solution, hydrogen peroxide, untreated sample, and under the influence of temperature and light, were determinated by the developed UHPLC method. Also, based on the developed method, their structures and possible pathways of fragmentation were assumed. Drylab software was used to determine the optimal conditions of chromatographic separation method (column temperature, composition of mobile phase, time gradient, pH).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:817288
Study programmeTitle: Applied Chemistry - Graduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry
Course: Applied organic chemistry
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra primijenjene kemije (magistar/magistra primijenjene kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-10-29 11:39:53