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master's thesis
Interakcija modelnih membrana s flavonoidima ugrađenim u magnetske nanočestice pod utjecajem vanjskog magnetskog polja
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. urn:nbn:hr:149:060572
Mandić, Lucija
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

Cite this document

Mandić, L. (2018). Interakcija modelnih membrana s flavonoidima ugrađenim u magnetske nanočestice pod utjecajem vanjskog magnetskog polja (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:060572

Mandić, Lucija. "Interakcija modelnih membrana s flavonoidima ugrađenim u magnetske nanočestice pod utjecajem vanjskog magnetskog polja." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:149:060572

Mandić, Lucija. "Interakcija modelnih membrana s flavonoidima ugrađenim u magnetske nanočestice pod utjecajem vanjskog magnetskog polja." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:149:060572

Mandić, L. (2018). 'Interakcija modelnih membrana s flavonoidima ugrađenim u magnetske nanočestice pod utjecajem vanjskog magnetskog polja', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:149:060572

Mandić L. Interakcija modelnih membrana s flavonoidima ugrađenim u magnetske nanočestice pod utjecajem vanjskog magnetskog polja [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2018 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:149:060572

L. Mandić, "Interakcija modelnih membrana s flavonoidima ugrađenim u magnetske nanočestice pod utjecajem vanjskog magnetskog polja", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:149:060572

Metadata
TitleInterakcija modelnih membrana s flavonoidima ugrađenim u magnetske nanočestice pod utjecajem vanjskog magnetskog polja
Title (english)Interaction of model lipid membrane with flavonoids encapsulated within magnetic nanoparticles within external applied magnetic fields
AuthorLucija Mandić
MentorHelena Otmačić Ćurković (mentor)
Committee memberHelena Otmačić Ćurković (predsjednik povjerenstva)
Committee memberEmi Govorčin Bajsić (član povjerenstva)
Committee memberSuzana Šegota (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2018-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Applied Chemistry
Abstract
U okviru ovog rada provedena je strukturna, morfološka i fizičko-kemijska karakterizacija magnetskih Fe3O4 mezoporoznih nanočestica sljedećim tehnikama: rendgenskom difrakcijom na polikristalnom uzorku (XRPD), pretražnom elektronskom mikroskopijom (SEM), Brunauer-Emmett-Teller (BET) analizom, UV/Vis spektrofotometrijom te infracrvenom spektroskopijom (FTIR). Provedena su ispitivanja s tri strukturno različita flavonoida (kvercetin, miricetin i miricitrin) koji su ugrađeni u Fe3O4 nanočestice. Količina ugrađenih flavonoida u nanočestice određena je spektrofotometrijskim mjerenjima apsorbancije supernatanta pomoću UV/Vis spektrofotometra. Nakon ugradnje flavonoida u mezoporozne nanočestice, praćena je kinetika njihova otpuštanja iz nanočestica u ovisnosti o eksternoj stimulaciji, tj. pri normalnim uvjetima, pod utjecajem permanentnog i oscilirajućeg magnetskog polja. Promjena strukturnih i morfoloških svojstava modelnih membrana inducirana njihovom reakcijom s flavonoidima, otpuštanim iz nanočestica pri različitim eksternim uvjetima, prije i poslije induciranog oksidativnog stresa istražena je mikroskopijom atomskih sila (AFM). Učinkovitost ugrađenih flavonoida u zaštiti modelnih membrana od oksidativnog stresa te njihova zaštitna uloga potvrđena je metodom dinamičkog raspršenja svjetlosti (DLS) te mjerenjima zeta potencijala.
Abstract (english)
The structural, morphological and physico-chemical characterizations of mesoporous magnetic Fe3O4 nanoparticles were conducted within the framework of this research using several techniques: X-ray diffraction (XRPD), scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) analysis, UV/Vis spectrophotometry and infrared spectroscopy (FTIR). The research was performed using three different flavonoids (quercetin, myricetin and myricitrin) and incorporated in Fe3O4 nanoparticles. The quantity of incorporated flavonoids in nanoparticles was determined by spectrophotometric measurment of supernatant with UV/Vis spectrophotometer. After incorporation of flavonoid within nanoparticles, their release profiles were followed by during the experiment the focus was on releasing the flavonoids from the nanoparticles at the standard conditions and in permanent and oscillating magnetic field using UV/Vis spectrophotometry. Changing the structural and morphological properties of model membranes induced by their reaction with flavonoids, laid off from the nanoparticles in different extreme conditions, before and after induced oxidative stress was investigated by atomic force microscopy. The efficiency of embedded flavonoids in protecting of model membranes from oxidative stress and their protective role was confirmed by dynamic light scattering and zeta potential measurements.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:060572
Study programmeTitle: Materials Science and Engineering - Graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-11-02 13:18:22