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undergraduate thesis
Oksidacija diola u prisustvu oksidoreduktaza
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. urn:nbn:hr:149:833725
Jelačić, Jelena
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

Cite this document

Jelačić, J. (2018). Oksidacija diola u prisustvu oksidoreduktaza (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:833725

Jelačić, Jelena. "Oksidacija diola u prisustvu oksidoreduktaza." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:149:833725

Jelačić, Jelena. "Oksidacija diola u prisustvu oksidoreduktaza." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2018. https://urn.nsk.hr/urn:nbn:hr:149:833725

Jelačić, J. (2018). 'Oksidacija diola u prisustvu oksidoreduktaza', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:149:833725

Jelačić J. Oksidacija diola u prisustvu oksidoreduktaza [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2018 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:149:833725

J. Jelačić, "Oksidacija diola u prisustvu oksidoreduktaza", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:149:833725

Metadata
TitleOksidacija diola u prisustvu oksidoreduktaza
Title (english)Diol oxidation in the presence of oxidoreductases
AuthorJelena Jelačić
MentorZvjezdana Findrik Blažević (mentor)
Committee memberZvjezdana Findrik Blažević (predsjednik povjerenstva)
Committee memberMartina Sudar (član povjerenstva)
Committee memberMarija Vuković Domanovac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2018-09-10, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Reaction Engineering
Abstract
U ovom radu istražena je enzimatska oksidacija alkohola katalizirana enzimom galaktoza oksidazom. Zanimala nas je specifična aktivnost galaktoza oksidaze prema alkoholnom supstratu, diolu. Provedeno je mjerenje utjecaja koncentracije diola, bakrovih iona i kisika na aktivnost enzima. Kao supstrati su korišteni racemična smjesa diola, ali i čisti R- i S- izomeri diola, kako bi se pokazalo je li enzim enantioselektivan. Postojanje produkta enzimatske reakcije oksidacije alkohola ispitano je kapljevinskom kromatografijom visoke djelotvornosti i kombinirane tehnike kapljevinske kromatografije i masene spektrometrije. Provedena je i kaskadna reakcija u kojoj je oksidacija alkohola kombinirana s aldolnom adicijom uz enzim D-fruktoza-6-fosfat aldolazu (FSA).
Abstract (english)
In this work, enzymatic alcohol oxidation catalyzed by galactose oxidase was studied. We were interested in the specific activity of galactose oxidase to the alcohol substrate. Measurement of the effect of diol, copper ions and oxygen concentration on enzyme activity was performed. The racemic alcohol was tested as substrate, but also the R- and S-isomers. The existence of enzymatic reaction products was tested by high-performance liquid chromatography and combined liquid chromatography and mass spectrometry techniques. Cascade reactions of alcohol oxidation and aldol addition to D-fructose-6-phosphate aldolase (FSA) were also performed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:833725
ProjectNumber: 635595
Title: Sustainable industrial processes based on a C-C bond-forming enzyme platform
Acronym: CARBAZYMES
Leader: Zvjezdana Findrik Blažević
Jurisdiction: eu
Funder: EC
Funding stream: H2020
Study programmeTitle: Applied Chemistry - Undergraduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry
Course: Advanced materials and technologies
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2021-06-24 12:50:44