The main objective of this thesis is to determine if selected deep eutectic solvents are better for the extraction of polyphenols from herbal material than most commonly used conventional solvents. Conventional solvents which are used in many different industries are attempted to be replaced because of their volatility, toxicity, flammability and price. The selected groups of polyphenols (phenolic acids and flavonoids) were extracted from two different herbs (elderberry and rosemary), i.e. from two different parts of those herbs (milled dry elderberry flowers and milled dry rosemary leaves) according to European pharmacopoeia regulations and quantitatively determined by the spectrophotometric method. The presence of phenol acids and flavonoids is confirmed by the thin layer chromatography Eight different solvents were compared considering the extraction of polyphenol compounds (distilled water, 70 % ethanol, 100 % ChCl:EG = 1:3, 70 % ChCl:EG = 1:3, 50 % ChCl:EG = 1:3, 100 % ChCl:Gly = 1:3, 70 % ChCl:Gly = 1:3, 50 % ChCl:Gly = 1:3). Unlike conventional solvents, deep eutectic solvents based on choline chloride, ethylene glycol and glycerol selected for this thesis belong to the group of green solvents. The impact of proton donor group on the extraction of polyphenol compounds is examined. The results of this study show that the deep eutectic solvents with 50 % water content are very efficient in extracting phenolic acids such as rosemary acid and chlorogenic acid, while all used solvents exhibit negligible extraction of flavonoids from plants in comparison with 70 % ethanol.