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master's thesis
Validacija preliminarnog testa na krv
Split: University of Split, University Department for Forensic Sciences, 2021. urn:nbn:hr:227:505772
Belobrajdić, Mia
University of Split
University Department of Forensic Sciences

Institutional repository: Repository of University Department for Forensic Sciences

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Belobrajdić, M. (2021). Validacija preliminarnog testa na krv (Master's thesis). Split: University of Split, University Department of Forensic Sciences. Retrieved from https://urn.nsk.hr/urn:nbn:hr:227:505772

Belobrajdić, Mia. "Validacija preliminarnog testa na krv." Master's thesis, University of Split, University Department of Forensic Sciences, 2021. https://urn.nsk.hr/urn:nbn:hr:227:505772

Belobrajdić, Mia. "Validacija preliminarnog testa na krv." Master's thesis, University of Split, University Department of Forensic Sciences, 2021. https://urn.nsk.hr/urn:nbn:hr:227:505772

Belobrajdić, M. (2021). 'Validacija preliminarnog testa na krv', Master's thesis, University of Split, University Department of Forensic Sciences, accessed 06 November 2024, https://urn.nsk.hr/urn:nbn:hr:227:505772

Belobrajdić M. Validacija preliminarnog testa na krv [Master's thesis]. Split: University of Split, University Department of Forensic Sciences; 2021 [cited 2024 November 06] Available at: https://urn.nsk.hr/urn:nbn:hr:227:505772

M. Belobrajdić, "Validacija preliminarnog testa na krv", Master's thesis, University of Split, University Department of Forensic Sciences, Split, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:227:505772

Metadata
TitleValidacija preliminarnog testa na krv
Title (english)Presumptive blood test validation
AuthorMia Belobrajdić
MentorDamir Marjanović (mentor)
MentorJosip Crnjac (komentor)
Committee memberSnježana Štambuk (predsjednik povjerenstva)
Committee memberIvan Jerković (član povjerenstva)
Committee memberJosip Kasum (član povjerenstva)
GranterUniversity of Split
University Department of Forensic Sciences
Split
Defense date and country2021-09-22, Croatia
Scientific / art field,
discipline and subdiscipline		INTERDISCIPLINARY AREAS OF KNOWLEDGE
Cognitive Science (Natural, Technical, Biomedical and Healthcare, Social and Humanistic Sciences)
Abstract
Cilj: Cilj ovog rada je bio utvrditi je li imunokromatografski test SERATEC HemDirect visoko specifičan za forenzičku identifikaciju ljudske krvi, odnosno postoje li životinje čija bi krv pokazala lažno pozitivan rezultat na ovom testu. U ovom istraživanju ispitala se osjetljivost i specifičnost testa kada je izložen punoj i razrijeđenoj krvi čovjeka i različitih životinjskih vrsta. Metode: Istraživanje je obuhvatilo osam uzoraka venske krvi, čovjek i sedam različitih životinjskih vrsta. Svi uzorci krvi analizirani su testom SERATEC HemDirect. Pripremljena je serija razrjeđenja (1/4, 1/8, 1/10, 1/16, 1/20, 1/24 te 1/40 samo za krv čovjeka) iz pune krvi dodavanjem dvostruko destilirane vode. Nakon što je napravljena serija razrjeđenja, 50 μL uzorka pune krvi i svakog razrjeđenja naneseno je na pamučnu bijelu tkaninu i ostavljeno da se osuši na sobnoj temperaturi. Nakon sušenja, 1/4 dijela uzorka izrezana je i ekstrahirana u HemDirect puferu 30 minuta. Po isteku protokolom propisanih 30 minuta dodale su se tri kapi svakog uzorka u jažicu za ispitivanje te su se rezultati očitavali i bilježili nakon 10 minuta. Rezultati: Test SERATEC HemDirect pokazao je pozitivne rezultate u svim analiziranim uzorcima ljudske krvi (puna krv i razrjeđenja 1/4, 1/8, 1/10, 1/16, 1/20, 1/24, 1/40). U analiziranim uzorcima krvi svih životinjskih vrsta test je pokazao negativne rezultate. Zaključak: Temeljem dobivenih rezultata zaključeno je da se SERATEC HemDirect kao brz i pouzdan test može koristiti za identifikaciju ljudske krvi u laboratoriju i na mjestu počinjenja kaznenog djela.
Abstract (english)
Aim: The aim of this study was to determine whether the SERATEC HemDirect immunochromatographic test is highly specific for forensic identification of human blood, and whether there are animals whose blood would show a false positive result on this test. In this study, the sensitivity and specificity of the test when exposed to whole and diluted blood of human and various animal species were examined. Methods: The study included eight samples of venous blood, human and seven different animal species. All blood samples were analyzed by the SERATEC HemDirect test. A dilution set (1/4, 1/8, 1/10, 1/16, 1/20, 1/24, and 1/40 for human blood only) was prepared from whole blood by adding double destilled water. A 50 μL sample of whole blood and each dilution were placed on a white cotton cloth and left to dry at room temperature. After drying, 1/4 of the sample was cut out and extracted in HemDirect buffer for 30 minutes. After extraction, three drops of each sample were added to the test well and the results were read and recorded after 10 minutes. Results: The SERATEC HemDirect test showed positive results in all analyzed human blood samples (whole blood and dilutions 1/4, 1/8, 1/10, 1/16, 1/20, 1/24, 1/40). In the analyzed blood samples of all animal species, the test showed negative results. Conclusion: Based on the obtained results, it was concluded that SERATEC HemDirect as a fast and reliable test can be used for identification of human blood in the laboratory and at the crime scene.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:227:505772
Study programmeTitle: Forensic Sciences
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra forenzike (magistar/magistra forenzike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) icon
Created on2021-10-15 07:27:02