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master's thesis
Utjecaj starosti traga krvi na uspješnost utvrđivanja porijekla krvi primjenom preliminarnog testa za krv
Split: University of Split, University Department for Forensic Sciences, 2021. urn:nbn:hr:227:327226
Vugdalić, Dženita
University of Split
University Department of Forensic Sciences

Institutional repository: Repository of University Department for Forensic Sciences

Cite this document

Vugdalić, Dž. (2021). Utjecaj starosti traga krvi na uspješnost utvrđivanja porijekla krvi primjenom preliminarnog testa za krv (Master's thesis). Split: University of Split, University Department of Forensic Sciences. Retrieved from https://urn.nsk.hr/urn:nbn:hr:227:327226

Vugdalić, Dženita. "Utjecaj starosti traga krvi na uspješnost utvrđivanja porijekla krvi primjenom preliminarnog testa za krv." Master's thesis, University of Split, University Department of Forensic Sciences, 2021. https://urn.nsk.hr/urn:nbn:hr:227:327226

Vugdalić, Dženita. "Utjecaj starosti traga krvi na uspješnost utvrđivanja porijekla krvi primjenom preliminarnog testa za krv." Master's thesis, University of Split, University Department of Forensic Sciences, 2021. https://urn.nsk.hr/urn:nbn:hr:227:327226

Vugdalić, Dž. (2021). 'Utjecaj starosti traga krvi na uspješnost utvrđivanja porijekla krvi primjenom preliminarnog testa za krv', Master's thesis, University of Split, University Department of Forensic Sciences, accessed 11 October 2024, https://urn.nsk.hr/urn:nbn:hr:227:327226

Vugdalić Dž. Utjecaj starosti traga krvi na uspješnost utvrđivanja porijekla krvi primjenom preliminarnog testa za krv [Master's thesis]. Split: University of Split, University Department of Forensic Sciences; 2021 [cited 2024 October 11] Available at: https://urn.nsk.hr/urn:nbn:hr:227:327226

Dž. Vugdalić, "Utjecaj starosti traga krvi na uspješnost utvrđivanja porijekla krvi primjenom preliminarnog testa za krv", Master's thesis, University of Split, University Department of Forensic Sciences, Split, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:227:327226

Metadata
TitleUtjecaj starosti traga krvi na uspješnost utvrđivanja porijekla krvi primjenom preliminarnog testa za krv
Title (english)The effect of blood stain aging on determination of blood origin using presumptive blood test
AuthorDženita Vugdalić
MentorDamir Marjanović (mentor)
MentorJosip Crnjac (komentor)
Committee memberSnježana Štambuk (predsjednik povjerenstva)
Committee memberIvan Jerković (član povjerenstva)
Committee memberJosip Kasum (član povjerenstva)
GranterUniversity of Split
University Department of Forensic Sciences
Split
Defense date and country2021-09-22, Croatia
Scientific / art field,
discipline and subdiscipline		INTERDISCIPLINARY AREAS OF KNOWLEDGE
Cognitive Science (Natural, Technical, Biomedical and Healthcare, Social and Humanistic Sciences)
Abstract
Cilj rada: Utvrditi utjecaj starosti krvnog traga na mogućnost određivanja porijekla krvnog traga i odrediti daju li puna krv i krv razrijeđena 20 puta, testirane preliminarnim testom u vremenskom razdoblju od sedam dana, iste rezultate. Metode: Prikupljena je ljudska i životinjska krv (krv goluba). Testirani su uzorci pune ljudske i životinjske krvi, te ljudske i životinjske krvi razrijeđene 20 puta. Krvni uzorci naneseni su u obliku krvne mrlje na čistu tkaninu u volumenu od 50 μL uzorka pune/razrijeđene ljudske/životinjske krvi. Nakon sušenja tkanine izrezana je ¼ od svake krvne mrlje te je stavljena u pufer Seratec HemDirect testa na 30 minuta. Nakon 30 minuta, na testnu pločicu nanošene su tri kapi pufera u kojem je prethodno ostavljen uzorak (cca 120 μL). Rezultati su očitavani 10 minuta nakon stavljanja uzorka na testnu pločicu. Za svaki od četiri vrste uzoraka testiranje je ponavljano svaka 24 h, počevši od nultog uzorka. Rezultati: Puna i razrijeđena ljudska krv dale su pozitivan rezultat na svakom od testova. Puna i razrijeđena životinjska krv dale su negativan rezultat na svim testovima. Zaključak: Iako se tijekom vremena u tragu krvi događaju kemijske promjene, Seratec HemDirect test pokazuje visoku osjetljivost na ljudsku krv, a na životinjsku krv ne reagira, te je kao takav upotrebljiv u forenzičnoj istrazi kod ispitivanja prisustva ljudske krvi u spornom tragu.
Abstract (english)
Aims: To determine the influence of blood trace age on the possibility of determining the origin of blood trace and to determine whether the whole blood and blood diluted 20 times, both tested by preliminary test over a period of seven days, give the same results. Methods: Human and animal blood (pigeon blood) was collected. Samples of the whole human and animal blood, and human and animal blood diluted 20 times, were tested. Blood samples were applied in the form of a blood stain to a clear cloth in a volume of 50 μL of whole/diluted human/animal blood sample. After drying, the cloth was cut from each blood stain and placed in Seratec HemDirect buffer for 30 minutes. After 30 minutes, three drops of buffer (approximately 120 μL) in which the sample was previously left were applied to the test plate. The results were read 10 minutes after placing the samples on the test plate. For each of the four sample types, testing was repeated every 24 h, starting from the zero – sample. Results: Whole and diluted human blood gave a positive result on each of the tests. Whole and diluted animal blood gave a negative result on all tests. Conclusion: Although chemical changes occur in the blood traces over time, the Seratec HemDirect test shows high sensitivity to the human blood and does not respond to animal blood, and as such is useful in forensic investigation when examining the presence of human blood in the trace of interest.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:227:327226
Study programmeTitle: Forensic Sciences
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra forenzike (magistar/magistra forenzike)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2021-10-15 07:31:17