prikaz prve stranice dokumenta Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems
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Scientific paper - Original scientific paper
Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems
International Journal of Legal Medicine, 133 (2019), 5; 1369-1380. https://doi.org/10.1007/s00414-019-02110-4
Zavala, Elena I.; Rajagopal, Swetha; Perry, George H.; Kruzic, Ivana; Bašić, Željana; Parsons, Thomas J.; Holland, Mitchell M.

Institutional repository: Repository of University Department for Forensic Sciences

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Zavala, E. I., Rajagopal, S., Perry, G. H., Kruzic, I., Bašić, Ž., Parsons, T. J. & Holland, M. M. (2019). Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems. International Journal of Legal Medicine, 133. (5), 1369-1380. doi: 10.1007/s00414-019-02110-4

Zavala, Elena I., et al. "Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems." International Journal of Legal Medicine, vol. 133, no. 5, 2019, pp. 1369-1380. https://doi.org/10.1007/s00414-019-02110-4

Zavala, Elena I., Swetha Rajagopal, George H. Perry, Ivana Kruzic, Željana Bašić, Thomas J. Parsons and Mitchell M. Holland. "Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems." International Journal of Legal Medicine 133, no. 5 (2019): 1369-1380. https://doi.org/10.1007/s00414-019-02110-4

Zavala, E. I., et al. (2019) 'Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems', International Journal of Legal Medicine, 133(5), pp. 1369-1380. doi: 10.1007/s00414-019-02110-4

Zavala EI, Rajagopal S, Perry GH, Kruzic I, Bašić Ž, Parsons TJ, and sur.. Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems. International Journal of Legal Medicine [Internet]. 2019 [cited 2024 July 24];133(5):1369-1380. doi: 10.1007/s00414-019-02110-4

E. I. Zavala, et al., "Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems", International Journal of Legal Medicine, vol. 133, no. 5, pp. 1369-1380, 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:227:769452. [Accessed: 24 July 2024]

Metadata
Title (english)Impact of DNA degradation on massively parallel sequencing-based autosomal STR, iiSNP, and mitochondrial DNA typing systems
AuthorElena I. Zavala
AuthorSwetha Rajagopal
AuthorGeorge H. Perry
AuthorIvana Kruzic
AuthorŽeljana Bašić
AuthorThomas J. Parsons
AuthorMitchell M. Holland
Author's institutionUniversity of Split
University Department of Forensic Sciences
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Scientific / art field,
discipline and subdiscipline		HUMANISTIC SCIENCES
Ethnology and Anthropology
Abstract (english)
Biological samples, including skeletal remains exposed to environmental insults for extended periods of time, exhibit increasing levels of DNA damage and fragmentation. Human forensic identification methods typically use a combination of mitochondrial (mt) DNA sequencing and short tandem repeat (STR) analysis, which target segments of DNA ranging from 80 to 500 base pairs (bps). Larger templates are often unavailable as skeletal samples age and the associated DNA degrades. Single-nucleotide polymorphism (SNP) loci target shorter templates and may serve as a solution to the problem. Recently developed assays for STR and SNP analysis using a massively parallel sequencing approach, such as the ForenSeq kit (Verogen, San Diego, CA), offer a means for generating results from degraded samples as they target templates down to 60 to 170 bps. We performed a modeling study that demonstrates that SNPs can increase the significance of an identification when analyzing DNA down to an average size of 100 bps for input amounts between 0.375 and 1 ng of nuclear DNA. Observations from this study were then compared with human skeletal material results (n = 14, ninth to eighteenth centuries), which further demonstrated the utility of the ForenSeq kit for degraded samples. The robustness of the Promega PowerSeq™ Mito System was also tested with human skeletal remains (n = 70, ninth to eighteenth centuries), resulting in successful coverage of 99.29% of the mtDNA control region at 50× coverage or more. This was accompanied by modifications to a mainstream DNA extraction technique for skeletal remains that improved recovery of shorter templates.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleInternational Journal of Legal Medicine
Numberingvol. 133, no. 5, pp. 1369-1380
p-ISSN0937-9827
e-ISSN1437-1596
DOIhttps://doi.org/10.1007/s00414-019-02110-4
URN:NBNurn:nbn:hr:227:769452
Publication2019
Document URLhttp://link.springer.com/10.1007/s00414-019-02110-4
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-06-10 12:41:10