The vast majority of modern components and structures that are subjected to dynamic loads and consequently to the fatigue, are made of standard ductile cast iron EN-GJS-400-18-LT. Main reason for this is the fact that this material is characterized by high elasticity and dynamic endurance which is accomplished by ferritic microstructural matrix encompassing graphite nodules. This material was used for making the hub of KONČAR-1MW windmill, as well as in other Končar's product components, such as train, trams, power equipment, etc. For this reason the research of deformation processes of this material represents a significant interest from the Croatian industry. Since the influence of microstructure on the mechanical behaviour of ductile cast iron EN-GJS-400-18-LT is not sufficiently investigated, the aim of this study was to experimentally investigate the influence of microstructure on the process of damage initiation and growth as well as to develop a constitutive model with an appropriate numerical algorithm to simulate the deformation processes of materials based on the experimental data. Aiming to describe the influence of microstructure of the mechanical behaviour of materials, this paper analyses four different series of materials obtained by different casting technologies. Characterization of graphite morphology and material matrix microstructure was performed on fifty samples for each series of ductile cast iron. The conducted experiments included monotonic tensile tests, symmetric and asymmetric cyclic test with hysteresis loops, fracture toughness, and fatigue crack growth tests, for each of the four series of ductile cast iron. For the detection and measurement of fatigue crack growth a new method using an ARAMIS optical system has been developed. Experimental results show that the size, shape and distribution of graphite nodules have no significant effect on the cyclic hardening, but it plays a major role in the processes of crack initiation and propagation. It was concluded that the larger, irregular nodules, adversely affect the fatigue behaviour when compared with smaller, more regular nodules. Furthermore, the results show that the pearlite microstructure phase does not significantly affect the fatigue life if its share doesn't exceed 10%. In the frame of numerical modelling, the existing algorithm for analysing problems of cyclic plasticity of the shell structure, was adapted to the three-dimensional constitutive model and was implemented in the software package ABAQUS. The accuracy of the numerical algorithm was verified by comparison with the obtained experimental results on specimens with and without notch which were used to develop a constitutive model. For cyclic loading cases this allowed more accurate description of stress and strain fields, as well as processes of crack initiation and propagation in the material.